Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
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This image is from an open access article distributed under the terms of the.

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.