RAT ANTI TUBULIN ALPHA MCA78G from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI TUBULIN ALPHA

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RAT ANTI TUBULIN ALPHA

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
.
This image is from an open access article distributed under the terms of the.
RAT ANTI TUBULIN ALPHA

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RAT ANTI TUBULIN ALPHA

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RAT ANTI TUBULIN ALPHA

Published customer image:
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.

From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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RAT ANTI TUBULIN ALPHA

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Bio-Rad (Formerly AbD Serotec)'s RAT ANTI TUBULIN ALPHA is a Rat monoclonal antibody. It reacts with Homo Sapiens, Human, Mouse, Amoeba/Protozoa, Drosophila/Arthropod, Plant, and Yeast/Fungi. This antibody has been shown to work in applications such as: EIA, Immunoassay, Histology, ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - frozen, Radioimmunoassay, and Western Blot.

Product Specs
ItemRAT ANTI TUBULIN ALPHA
CompanyBio-Rad
Price $594.00Supplier Page View Company Product Page
Catalog NumberMCA78G
ImmunogenYeast tubulin
TargetTUBULIN ALPHA
CloneYOL1/34
ApplicationsWestern Blotting,Immunofluorescence,Immunohistology - Frozen,ELISA,Radioimmunoassays
Quantity0.5 mg
FormatPurified IgG - liquid
HostRat
ReactivityDrosophila,Human,Arabidopsis thaliana,Saccharomyces,Platyzoma,Ashbya,Mouse,Naegleria,Asplenium nidus,Seagrass (Cymodocea nodosa),Rye (Secale cereale L.), Yeast
IsotypeIgG2a
ClonalityMonoclonal Antibody
ConcentrationIgG concentration 1.0mg/ml

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Questions and Answers

What species does this antibody work with in immunofluorescence applications?

The rat anti-tubulin antibody has been shown to work in immunofluorescence applications with various species, including yeast (Saccharomyces cerevisiae) and Drosophila, as indicated in multiple studies where it was utilized for tubulin staining.

Can this antibody be used for Western blotting, and if so, what are the recommended dilutions?

Yes, the rat anti-tubulin antibody has been successfully used in Western blotting. In one study, it was used at a dilution of 1:500 for immunoblot analysis, demonstrating its effectiveness in detecting α-tubulin.

Is there any evidence of this antibody being used in studies related to meiosis?

Yes, the rat anti-tubulin antibody has been referenced in studies investigating meiotic processes, such as in the research by Chen et al. (2017), where it was employed to visualize tubulin during meiotic stages in budding yeast.

How does this antibody contribute to understanding cellular processes in yeast?

The rat anti-tubulin antibody is crucial for studying microtubule dynamics and organization during cellular processes such as meiosis and mitosis in yeast, as evidenced by its application in various studies that explore the regulation of spindle orientation and chromosome segregation.

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(1) Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complexJ Cell BiolMay 4, 2026Noah N. Turner, Ziguo Zhang, Jing Yang, Kyle W. Muir, Stephen H. McLaughlin, Tomos Morgan, David Barfordantibodies, respectively, were used. To detect alpha tubulin as a loading control, anti-α-tubulin (MCA78G; Bio-Rad, rat monoclonal) antibody was used. Membranes were washed four times with PBS-T before incubation

(2) Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategyeLifeApril 9, 2026Lori B Koch, Tiasha Ghosh, Christos Spanos, Adele L Marstonby indirect immunofluorescence as described in Barton et al., 2022. A rat anti-tubulin primary antibody (Bio-Rad Cat# MCA78G, RRID:AB_325005) at 1:50 dilution and an anti-rat FITC-conjugated secondary antibody (Jackson ImmunoResearch

(3) Large-scale identification of plasma membrane repair proteins revealed spatiotemporal cellular responses to plasma membrane damageeLifeMarch 10, 2026Yuta Yamazaki, Keiko Kono(Santa Cruz Biotechnology, sc-40), anti-GFP (Roche/Merck, 11814460001), or anti-α-tubulin (Bio-Rad, MCA78G) antibodies. Statistical analysis Dunnett’s multiple comparison test, Welch’s t-test, and Mann-Whitney U test

(4) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. GladfelterHybridization mix was vortexed for 30 s to mix. Finally, a 1:500 dilution of anti-tubulin antibody (Bio-Rad, MCA78G) was added to Cln3 reactions and tapped to mix. This mixture was added to the cell pellet and sharply

(5) Targeting the splicing factor CWC22 induces mitotic slippage through repression of BubR1 expression and CDK1 activity in cancer cellsJournal of Biological ChemistryMarch 1, 2026Ryuzaburo Yuki, Youhei Saito, Yuji Nakayamaimmunoblotting (IB), and FC: rat monoclonal anti-α-tubulin (IF, 1:800 dilution; IB, 1:4000 dilution; MCA78G; Bio-Rad), rabbit polyclonal anti-CWC22 (IB, 1:1000 dilution; 26898-1-AP, Proteintech Group, Inc), rabbit

(6) RNAi reveals a unique set of kinesins mediating chloroplast motility in the giant cytoplasm of Bryopsis (Ulvophyceae), a coenocytic green algaJournal of PhycologyDecember 6, 2025Harumi A. Ogawa, Kanta K. Ochiai, Maki Shirae‐Kurabayashi, Gohta Goshimah at 25°C, followed by overnight rotation at 4°C with primary antibody (rat anti‐α‐tubulin—YOL1/34, MCA78G, Bio‐Rad—1:1000). Samples were washed three times with PBST and incubated with rotation overnight at

(7) Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasisJournal of Biological ChemistryDecember 1, 2025Yun-Yu Huang, Ya-Lan Chang, Yun Chen, Wei-Yu Chiang, Ai-Tao Chiang, Pang-Hung Hsu, Shu-Chun Teng(1:1000, sc-66048, Santa-cruz Biotechnology), FKBP5 (1:1000, GTX113438, GeneTex), α-tubulin (1:1000, MCA78G, Bio-Rad Laboratories Inc), FLAG (1:2000, F3165, Sigma-Aldrich), c-Myc (1:1000, #11667203001, Roche),

(8) Healthy live birth after microsurgical enucleation of tripronuclear human zygote derived from ICSI: a case reportJournal of Ovarian ResearchNovember 4, 2025Xiaoming Xu, Xiuhe Zuo, Shan Du, Chunlai Zhang, Haowen Xu, Yabing Luo, Ran Shi, Shumin Hu, Huan Shen, Yu Wang, et al.with 0.5% triton X-100 and 5% BSA (PBT-BSA). Oocytes were incubated with rat anti-α-tubulin antibody (MCA78G; Bio-Rad) at 10 µg/mL overnight at 4℃, washed in PBT-BSA five times for 3 min each and incubated with

(9) Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosisNature CommunicationsDecember 23, 2024Eirini Bellou, Agata P. Zielinska, Eike Urs Mönnich, Nina Schweizer, Antonio Z. Politi, Antonina Wellecke, Claus Sibold, Andreas Tandler-Schneider, Melina Schuhdilution (Antibodies Incorporated #15-234), rabbit anti-GFP (#A11122, Invitrogen), rat anti-alpha-tubulin (MCA78G, Bio-rad), mouse anti-TRF-2 (NB100-56506SS), goat anti-GFP (600-101-215; Rockland Immunochemicals),

(10) DeSUMOylating isopeptidase 1 participates in the faithful chromosome segregation and vincristine sensitivityFASEB journal : official publication of the Federation of American Societies for Experimental BiologyDecember 13, 2024Ikeda Y, Yuki R, Saito Y, Nakayama Y.Santa Cruz Biotechnology) antibodies; rat monoclonal anti‐α‐tubulin (IB, 1:4000; IF, 1:800; YOL1/34, MCA78G, Bio‐Rad, Hercules, CA, USA) and anti‐HA (IB, 1:1000; 3F10, 11 867 423 001, Roche, Basel, Switzerland)

(11) Phosphorylation of Ephexin4 at Ser-41 contributes to chromosome alignment via RhoG activation in cell divisionJournal of Biological ChemistryDecember 13, 2024Ryuji Yasutake, Hiroki Kuwajima, Ryuzaburo Yuki, Junna Tanaka, Youhei Saito, Yuji Nakayama1:1000; C-20, sc-6216, Santa Cruz Biotechnology) antibody, rat anti-α-tubulin (IF, 1:800; WB, 1:2000; MCA78G, Bio-Rad) antibody, and human anti-centromere antigen (IF, 1:400; HCT0100, ImmunoVision) were used.

(12) LAT1 supports mitotic progression through Golgi unlinking in an amino acid transport activity-independent mannerThe Journal of biological chemistryOctober 1, 2024Yanagida S, Yuki R, Saito Y, Nakayama Y.immunofluorescence (IF) and immunoblotting (IB): rat monoclonal anti-α-tubulin (IF, 1:800; IB, 1:4000; MCA78G, Bio-Rad), rabbit polyclonal anti-LAT1 (IB, 1:4000; #5347, Cell Signaling Technology), rabbit polyclonal

(13) Swi/Snf chromatin remodeling regulates transcriptional interference and gene repressionMolecular CellAugust 22, 2024Kaitlin Morse, Alena L. Bishop, Sarah Swerdlow, Jessica M. Leslie, Elçin Ünalα-V5 antibody (R960-25, Thermo Fisher Scientific) used at a concentration of 1:2,000; rat α-tubulin (MCA78G, Bio-Rad) used at a concentration of 1:10,000. Secondary antibodies: goat α-mouse or α-rabbit secondary

(14) Intrinsically disordered sequences can tune fungal growth and the cell cycle for specific temperaturesCurrent BiologyAugust 19, 2024Benjamin M. Stormo, Grace A. McLaughlin, Ameya P. Jalihal, Logan K. Frederick, Sierra J. Cole, Ian Seim, Fred S. Dietrich, Ashutosh Chilkoti, Amy S. GladfelterRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rat anti-Alpha Tubulin YOL1/34,Bio-rad,CAT# MCA78G RRID:AB_93477 Goat anti-Rat-Cy3,Millipore-Sigma,CAT # AP136C RRID:AB_11211234 Bacterial and virus

(15) Actin networks modulate heterogeneous NF-κB dynamics in response to TNFαeLifeAugust 7, 2024Francesca Butera, Julia E Sero, Lucas G Dent, Chris Bakalantibodies used were rabbit anti-p65/RELA NF-κB (Abcam #16502; 1:500), rat anti-α-tubulin (Bio-Rad #MCA78G; 1:1000), and rabbit anti-pFAK Tyr397 (Invitrogen #44-624G; 1:250). Secondary antibodies used were Alexa

(16) Exocytic plasma membrane flows remodel endoplasmic reticulum-plasma membrane tethering for septin collar assemblySci AdvMarch 15, 2024Shinju Sugiyama, Keiko Konoperformed with anti-mini AID (MBL, M214-3), anti-mCherry (Abcam, ab125096), or anti–tubulin α (Bio-Rad, MCA78G) antibodies. Fluorescence recovery after photobleaching The fluorescence in a square of 0.9 μm by

(17) Qualitative rather than quantitative phosphoregulation shapes the end of meiosis I in budding yeastThe EMBO JournalFebruary 6, 2024Dunja Celebic, Irem Polat, Véronique Legros, Guillaume Chevreux, Katja Wassmann, Sandra A TouatiWerven,N/A Antibodies,Antibodies,Antibodies Rat monoclonal anti- a-tubulin (YOL1/3),Bio-Rad,Cat# MCA78G Anti-rabbit IgG (HRP-conjugated),GE Healthcare,Cat# NA934 Phospho-MAPK/CDK Substrates (PXSP),Ozyme,Cat#

(18) Biomolecular condensates in fungi are tuned to function at specific temperaturesbioRxivNovember 30, 2023Benjamin M. Stormo, Grace A. McLaughlin, Logan K. Frederick, Ameya P. Jalihal, Sierra J Cole, Ian Seim, Fred S. Dietrich, Amy S. GladfelterPBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed, blocked again and stained with secondary antibody (Goat-Anti-Rat conjugated

(19) Swi/Snf Chromatin Remodeling Regulates Transcriptional Interference and Gene RepressionbioRxivApril 30, 2023Kaitlin Morse, Sarah Swerdlow, Elçin Ünalα-V5 antibody (R960–25, Thermo Fisher Scientific) used at a concentration of 1:2,000; rat α-tubulin (MCA78G, Bio-Rad) used at a concentration of 1:10,000. Secondary antibodies: goat α-mouse or α-rabbit secondary

(20) Meiotic resetting of the cellular Sod1 pool is driven by protein aggregation, degradation, and transient LUTI-mediated repressionJournal of Cell BiologyMarch 6, 2023Helen M. Vander Wende, Mounika Gopi, Megan Onyundo, Claudia Medrano, Temiloluwa Adanlawo, Gloria Ann Brartemperature. For tubulin immunofluorescence staining, rat α-tubulin primary antibody (RID AB_325005, MCA78G; Bio-Rad) was diluted 1:200 in 1X PBS-BSA, 5 μl of primary dilution was added to each well, and slides

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RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI TUBULIN ALPHA from Bio-Rad (Formerly AbD Serotec)

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