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Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to block CD200 interactions by incubation in vitro with murine BV2 cells.
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N44Q mutant involved in CD200-CD200R1 interaction and LPS-induced classical microglial activation. We established neuronal-microglia co-cultures to explore the effect of N44Q mutant. a, b LPS significantly increased iNOS mRNA and CD86 mRNA in BV2 cells. The addition of neurons attenuated LPS-induced classical microglial activation. Notably, the present of anti-CD200 antibody blocked the modulating effect of neurons. BV2 cells transfected with N44Q mutant exerted similar effects like anti-CD200 antibody (*p < 0.01; +p < 0.01; #p < 0.01, WT + LPS + neurons vs WT + LPS; **p < 0.01; N44Q + LPS + neurons vs N44Q + LPS; ++p < 0.01, N44Q + LPS + neurons + anti-CD200 antibody vs N44Q + LPS + neurons; ANOVA; n = 5). c, d IL-1β mRNA and TNF-α mRNA were examined. The LPS-induced effects were reversed in the presence of neurons. Importantly, the addition of anti-CD200 antibody abrogated the modulating effect of neurons, consistent with the cells transfected with N44Q mutant (*p < 0.01; +p < 0.01; #p < 0