RAT ANTI HUMAN INTERLEUKIN-2

Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 and FITC conjugated Rat anti Human IL-2 (MCA1553F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 and FITC conjugated rat IgG2a . Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 and FITC conjugated Mouse anti Human IL-2 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Rat anti Human IL-2 (MCA1553F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated rat IgG2a (MCA6005F). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IL-2 (MCA1553F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.