RAT ANTI MOUSE CD45 MCA1388 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE CD45
CompanyBio-Rad
Price $408.00Supplier Page View Company Product Page
Catalog NumberMCA1388
Quantity0.25 mg
ApplicationsImmunoprecipitation,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence
ConcentrationIgG concentration 0.5 mg/ml
FormatPurified IgG - liquid
ReactivityMouse
TargetCD45
IsotypeIgG1
CloneIBL-3/16
HostRat
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type C
NCBI Gene Aliasesloc, Cd45, L-CA, CD45R, T200, Lyt-4, B220, Ly-5
ClonalityMonoclonal Antibody

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Human and Mouse Alzheimer's Seeds Differentially Affect Amyloid Deposition and Microglia‐Dependent Plaque Response in Aged MiceAging CellMay 13, 2025Juana Andreo‐Lopez, Cristina Nuñez‐Diaz, Kelly Do Huynh, Marie Minh Thu Nguyen, Celia Da Cunha, Francisco J. Cantero‐Molina, Cynthia Campos‐Moreno, Stefania Zimbone, Francesco Bellia, Maria Laura Giuffrida, et al.monoclonal, 1:1000; ThermoScientific; Catalog #MN1020), CD45 (rat polyclonal, 1:500; Bio‐Rad; Catalog #MCA1388), CD68 (rabbit polyclonal, 1:1000; Abcam Cambridge, UK; Catalog #AB125212), GFAP (rabbit polyclonal,

(2) Delivery of CDNF by AAV-mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinson's diseaseScientific reportsJuly 17, 2024Nam J, Richie CT, Harvey BK, Voutilainen MH.(FA-11, 1:200, Biolegend, 137007, RRID:AB_10575299), rat anti-mouse CD45 (IBL-3/16, 1:200, Bio-Rad, MCA1388, RRID:AB_321729) and rabbit anti-Interleukin-1β (IL-1β, 1:500, Abcam, ab9722, RRID:AB_308765). The following

(3) Intracerebellar injection of monocytic immature myeloid cells prevents the adverse effects caused by stereotactic surgery in a model of cerebellar neurodegenerationJournal of neuroinflammationFebruary 14, 2024Del Pilar C, Garrido-Matilla L, Del Pozo-Filíu L, Lebrón-Galán R, Arias RF, Clemente D, Alonso JR, Weruaga E, Díaz D.ab16669, Abcam, Cambridge, UK), rat anti-CD16/CD32 (1:200; 553142, BD Biosciences), rat anti-CD45 (1:1000; MCA1388, Bio-Rad Laboratories, Hercules, CA, USA), rat anti-CD45R/B220 (1:200; 553089, BD Biosciences), goat

(4) Fetal CCL2 signaling mediates offspring social behavior and recapitulates effects of prenatal stressBrain, behavior, and immunityJanuary 1, 2024Chen HJ, Galley JD, Verosky BG, Yang FT, Rajasekera TA, Bailey MT, Gur TL.immunofluorescence staining include rat anti-mouse CD45 primary antibody (1:300; Bio-Rad, Hercules, CA; Catalog # MCA1388) and Alexa Fluor 488 donkey anti-rat secondary antibody (1:1000; Abcam, Cambridge, MA; Catalog # R37119). 1.2.9

(5) Antagonism of the brain P2X7 ion channel attenuates repeated social defeat induced microglia reactivity, monocyte recruitment and anxiety-like behavior in male miceBrain, Behavior, and ImmunityJanuary 1, 2024Rebecca G. Biltz, Samuel P. Swanson, Natalie Draime, Amara C. Davis, Wenyuan Yin, Ethan J. Goodman, Natalie R. Gallagher, Anindya Bhattacharya, John F. Sheridan, Jonathan P. Godbout019–19741), goat anti-mouse GFAP (1:500, Abcam, cat #ab53554), and rat anti-mouse CD45 (1:500; BioRad, cat#MCA1388). Sections were incubated overnight at 4°C. Next, sections were washed in PBS and incubated with an

(6) Divergent Spatial Learning, Enhanced Neuronal Transcription, and Blood–Brain Barrier Disruption Develop During Recovery from Post-Injury Sleep FragmentationNeurotrauma ReportsSeptember 27, 2023Zoe M. Tapp, Cindy Ren, Kelsey Palmer, Julia Kumar, Ravitej R. Atluri, Julie Fitzgerald, John Velasquez, Jonathan Godbout, John Sheridan, Olga N. Kokiko-Cochranfibrillary acidic protein (GFAP; 1:1000; NC0421235; Invitrogen, Carlsbad, CA); rat anti-mouse CD45 (1:1000; MCA1388; Bio-Rad Laboratories, Hercules, CA); and rabbit anti-mouse aquaporin-4 (AQP4; 1:2000; HPA014784; Sigma-Aldrich,

(7) Oleoylethanolamide Treatment Modulates Both Neuroinflammation and Microgliosis, and Prevents Massive Leukocyte Infiltration to the Cerebellum in a Mouse Model of Neuronal DegenerationInternational journal of molecular sciencesJune 2, 2023Pérez-Martín E, Pérez-Revuelta L, Barahona-López C, Pérez-Boyero D, Alonso JR, Díaz D, Weruaga E.USA), goat anti-CD206 (1:200; #AF2535, R&D Systems, Minneapolis, MN, USA), rat anti-CD45 (1:1000; #MCA1388, Bio-Rad Laboratories, Hercules, CA, USA), and rabbit anti-Iba1 (1:1000; #019-19741, Wako Pure Chemical

(8) Adventitial macrophage accumulation impairs perivascular nerve function in mesenteric arteries with inflammatory bowel diseaseFrontiers in physiologyJanuary 1, 2023Grunz EA, Jones BW, Lateef OM, Sen S, Wilkinson K, Joshi T, Boerman EM.(TH),mouse,MilliporeSigma,T2928,1:500,AB_477569 Protein Gene Product 9.5 (PGP 9.5),rabbit,ThermoFisher,PA5-32567,1:500,AB_2550032 CD45,rat,Biorad,MCA1388,1:250,AB_321729 CD68,mouse,ThermoFisher,14-0681-82,1:250,AB_2572857 CD163,rabbit,Bioss,Bs-2527R,1:250,AB_10856166 Major

(9) The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous mannerPLoS biologyJanuary 1, 2022Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, et al.of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250; #MCA1388, AbD Serotec), rabbit anti-P2Y12R (1:500; #55043AS, AnaSpec). After the incubation with the primaries,

(10) The Selective Loss of Purkinje Cells Induces Specific Peripheral Immune AlterationsFrontiers in Cellular NeuroscienceNovember 30, 2021Carlos del Pilar, Rafael Lebrón-Galán, Ester Pérez-Martín, Laura Pérez-Revuelta, Carmelo Antonio Ávila-Zarza, José Ramón Alonso, Diego Clemente, Eduardo Weruaga, David Díaz0.2% v/v Triton X-100, 5% v/v normal donkey serum, and the primary antibodies: rat anti-CD45 (1:1,000; MCA1388; Bio-Rad Laboratories, Hercules, CA, United States), rabbit anti-Iba1 (1:1,000, 019-19741, Wako Pure

(11) Extracellular Vesicles Secreted by Mesenchymal Stromal Cells Exert Opposite Effects to Their Cells of Origin in Murine Sodium Dextran Sulfate-Induced ColitisFrontiers in immunologyJanuary 1, 2021Tolomeo AM, Castagliuolo I, Piccoli M, Grassi M, Magarotto F, De Lazzari G, Malvicini R, Caicci F, Franzin C, Scarpa M, et al.(abcam) Mouse anti-Muc5ac,1:80,O.N. 4°C,45M1,MA512178 (Invitrogen) Rat anti-CD45,1:100,O.N. 4°C,IBL-3/16,MCA1388 (Biorad) "Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor 594",1:200,1 h 37°C,Polyclonal,A11005

(12) Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic miceBMC Veterinary ResearchDecember 1, 2020Liljana Markova, Nejc Umek, Simon Horvat, Admir Hadžić, Max Kuroda, Tatjana Stopar Pintarič, Vesna Mrak, Erika Cvetkofor leucocyte receptor-type tyrosine-protein phosphatase C (CD45) labelling with anti-CD45 antibody (MCA1388, Bio-Rad Laboratories Inc., San Francisco, CA, USA) and revealed by a secondary antibody P0260 (Dako,

(13) Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brainJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDecember 1, 2020Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, et al.Synaptic Systems), guinea pig anti-glutamine synthetase (1:500, #367 005, Synaptic Systems), rat anti-CD45 (1:250, #MCA1388, Bio-Rad), goat anti-ICAM-1 (1:250, #AF796, R&D Systems) and mouse anti-Kv2.1 (1:500, #75-014 NeuroMab).

(14) Plaque-associated myeloid cells derive from resident microglia in an Alzheimer's disease modelThe Journal of experimental medicineApril 6, 2020Reed-Geaghan EG, Croxford AL, Becher B, Landreth GE.included rabbit anti-RFP (1:500; Rockland; 600-401-379), rat anti-CD45 clone IBL-3/16 (1:500; Bio-Rad; MCA1388); biotinylated sheep anti-TREM2 (1:200; R&D Systems; BAF1729), rat anti-Dectin1 clone R1-8g7 (1:50;

(15) Inhibitory designer receptors aggravate memory loss in a mouse model of down syndromeNeurobiology of diseaseFebruary 1, 2020Hamlett ED, Ledreux A, Gilmore A, Vazey EM, Aston-Jones G, Boger HA, Paredes D, Granholm AE.Matched sections from various regions were incubated overnight at room temperature with anti-CD45 (#MCA1388, dil. 1:200, Bio-Rad, Hercules, CA). After washing, sections were incubated for one hour with secondary

(16) Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating DiseaseImmunityJanuary 14, 2020Werneburg S, Jung J, Kunjamma RB, Ha SK, Luciano NJ, Willis CM, Gao G, Biscola NP, Havton LA, Crocker SJ, et al.(clone 8/114B, Thermo Fisher Scientific, MA5–13473, 1:20), rat mAb α-CD45 (clone IBL-3/16, BioRad, MCA1388, 1:100), mouse mAb α-CD68 (clone KP1, Abcam, ab955, 1:200), rat mAb α-CD68 (clone FA-11, AbD Serotec,

(17) Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune ActivitiesImmunityFebruary 19, 2019Liu X, Nemeth DP, McKim DB, Zhu L, DiSabato DJ, Berdysz O, Gorantla G, Oliver B, Witcher KG, Wang Y, et al.RRID: AB_839504 Goat anti-lba1,Abeam,Cat# ab5076; RRID: AB_2224402 Rat anti-CD45,Abd Serotec,Cat# MCA1388; RRID: AB_321729 Rat anti-CD31,Biolegend,Cat# 102502; RRID: AB_312909 Armenian hamster anti-CD31,DSHB,Cat#:

(18) Microglia Promote Increased Pain Behavior through Enhanced Inflammation in the Spinal Cord during Repeated Social Defeat StressThe Journal of NeuroscienceFebruary 13, 2019Caroline M. Sawicki et al.rabbit anti-p2y12 (AS-55043A, AnaSpec) was combined with a 1:1000 dilution of rat anti-mouse CD45 (MCA1388, Bio-Rad) in PBS. Incubation of the primary antibody occurred overnight at 4°C. A 1:1000 dilution of

(19) Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neuronsBrain, behavior, and immunityFebruary 1, 2019Wong R, Lénárt N, Hill L, Toms L, Coutts G, Martinecz B, Császár E, Nyiri G, Papaemmanouil A, Waisman A, et al.(1:10,000, kindly gifted by Professor Daniel Anthony, University of Oxford, UK), rat anti-CD45 (1:250, #MCA1388, AbD Serotec, UK), goat-anti MPO (1:250, #AF3667, Novus Biologicals, UK), mouse anti-neurofilament-H

(20) Absence of TGFβ signaling in retinal microglia induces retinal degeneration and exacerbates choroidal neovascularizationeLifeJanuary 22, 2019Ma W, Silverman SM, Zhao L, Villasmil R, Campos MM, Amaral J, Wong WT.#05–710), CD74 (1:100, BD Biosciences, #555318), F4/80 (1:100, Bio-Rad, #MCA497), CD45 (1:100, Bio-Rad, #MCA1388), glutamine synthetase (1:200, Millipore, #MAB302), BRN3A (1:100, Santa Cruz, #SC31984), cone arrestin

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RAT ANTI MOUSE CD45

RAT ANTI MOUSE CD45

RAT ANTI MOUSE CD45 from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI MOUSE CD45 from Bio-Rad (Formerly AbD Serotec)

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