SHEEP ANTI RAT TGN38 AHP499G from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemSHEEP ANTI RAT TGN38
CompanyBio-Rad
Price $575.00Supplier Page View Company Product Page
Catalog NumberAHP499G
Quantity25 µg
ApplicationsWestern Blotting,Immunohistology - Frozen,Immunofluorescence
TargetTGN38
ReactivityRat, Mouse
FormatPurified IgG - liquid
ConcentrationIgG concentration 0.1 mg/ml
HostSheep
IsotypeIgG
NCBI Gene AliasesTgoln1, Ttgn1, Tgn38
ClonalityPolyclonal Antibody
NCBI Full Gene Nametrans-golgi network protein 2

Questions and Answers

Can this antibody be used for immunocytochemistry in my studies on Golgi apparatus proteins?

Yes, the sheep anti-TGN38 antibody (AHP499G) has been utilized in immunocytochemistry, as indicated in various studies, including those examining the localization of proteins in the Golgi apparatus of neurons and cardiac myocytes (, ).

What species does this antibody react with, and is it suitable for my rat models?

The sheep anti-TGN38 antibody is reactive with both rat and mouse species, making it suitable for use in experiments involving these models ().

Are there any specific applications where this antibody has been shown to be effective?

This antibody has been shown to work effectively in applications such as Western blotting, immunofluorescence, and immunohistochemistry, as evidenced by its use in studies investigating the Golgi apparatus and related signaling pathways (, ).

How has this antibody been utilized in research related to STING and Golgi signaling?

The sheep anti-TGN38 antibody has been employed to study the localization and function of STING at the trans-Golgi network, where it plays a critical role in innate immune signaling, as demonstrated in research examining STING clustering and its interactions with other proteins (, ).

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Tmem45b modulates itch via endoplasmic reticulum calcium regulationFront PhysiolDecember 10, 2025Sa-Shuang Wang, Chen Liang, Ruo-Lin Wang, Ze-Lin Sun, Peng-Yu Ren, Bin Wu, Juan-Juan Sun, Li Fu, Li-Zu Xiao, Wu-Ping Sun, et al.ab112995), Serca1 (Proteintech, 22361-1-AP), mitochondria-tracer (Beyotime, C1048), TGN38 (Bio-Rad, AHP499G), GFAP (Millipore, MAB3402), IBA1 (Abcam, ab5076). RNAscope ISH The probes and detection kit of RNAscope

(2) Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activationNature immunologyOctober 1, 2025Xiao N, Kogishi A, Radochonski L, Lei Y, Chen J.LAMP1 (sc-20011), and CD63 (sc-5275) were from Santa Cruz Biotechnology. Antibody against mouse TGN38 (AHP499G) was from Bio-Rad. Antibody against β-actin (PA1-16889) was from Pierce. Antibody against HA (MMS-101P)

(3) Subcellular activation of β-adrenergic receptors using a spatially restricted antagonistProceedings of the National Academy of SciencesOctober 1, 2024Federica Liccardo, Johannes Morstein, Ting-Yu Lin, Julius Pampel, Di Lang, Kevan M. Shokat, Roshanak Irannejad(ab221804) is from Abcam, mouse anti-a-Actinin antibody (7811) is from Sigma, and sheep anti-TGN38 antibody (AHP499G) is from Bio-Rad. Amersham ECL donkey anti-rabbit IgG (NA934V) and horseradish peroxidase-linked whole

(4) Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterolNature CommunicationsJanuary 11, 2024Haruka Kemmoku, Kanoko Takahashi, Kojiro Mukai, Toshiki Mori, Koichiro M. Hirosawa, Fumika Kiku, Yasunori Uchida, Yoshihiko Kuchitsu, Yu Nishioka, Masaaki Sawa, et al.Goat anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (SouthernBiotech); sheep anti-TGN38 (AHP499G, dilution 1:200) (Bio-Rad); mouse anti-GM130 (610823, dilution 1:4000) (BD Biosciences); Alexa 568-,

(5) Cardiac contraction and relaxation are regulated by distinct subcellular cAMP poolsNature chemical biologyJanuary 1, 2024Lin TY, Mai QN, Zhang H, Wilson E, Chien HC, Yee SW, Giacomini KM, Olgin JE, Irannejad R.(P9310S) is from New England Biolabs. Mouse anti-GM130 (610822) is from BD Biosciences. Sheep anti-TGN38 (AHP499G) is from Bio-Rad. Mouse anti-α-actinin (A7811) is from Sigma. Donkey anti-mouse IgG (H + L) highly cross-adsorbed

(6) Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neuronseLifeSeptember 11, 2023Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M.(rabbit polyclonal),Novus Biologicals,NBP1-90532,1:100 Antibody,anti-TGN38 (sheep polyclonal),Bio-Rad,AHP499G,1:100 Recombinant DNA reagent,pLenti-Syn(pr)-Cre-mCherry,"Wolzak et al., 2022",-, Recombinant DNA

(7) Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neuronsScientific reportsFebruary 18, 2023van Bommel DM, Toonen RF, Verhage M.,164 005,Guinea pig Giantin,1:200,BioLegend,909701,Rabbit GM130,1:500,BD,610822,Mouse TGN38,1:100,BioRad,AHP499G,Sheep Primary antibodies used for immunocytochemistry.

(8) A non-nucleotide agonist that binds covalently to cysteine residues of STINGCell Structure and FunctionFebruary 16, 2023Kentaro Matsumoto, Shenwei Ni, Hiroyuki Arai, Takashi Toyama, Yoshiro Saito, Takehiro Suzuki, Naoshi Dohmae, Kojiro Mukai, Tomohiko TaguchiGoat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G, dilution 1:200) (Bio-Rad); mouse anti-GM130 (610823, dilution 1:2000) (BD Biosciences). Reagents The

(9) Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organizationScientific reportsDecember 2, 2022van Bommel DM, Toonen RF, Verhage M.for immunocytochemistry: GM130 (1:500, BD, 610822), giantin (1:200, BioLegend, 909701), TGN38 (1:100, BioRad, AHP499G), TMEM87A (1:100, Novus Biologicals, NBP1-90532), ARL1 (1:50, Santa Cruz, sc-393785), HID1 (1:100, Novus Biologicals

(10) Interruption of post-Golgi STING trafficking activates tonic interferon signalingNature communicationsNovember 15, 2022Tu X, Chu TT, Jeltema D, Abbott K, Yang K, Xing C, Han J, Dobbs N, Yan N.microscopy imaging included Sting (19851-1-AP; Proteintech, 1:200), GM130 (610822; BD, 1:200), TGN38 (AHP499G; Bio-Rad, 1:200), Hsp60 (sc-13115; Santa Cruz, 1:200), PDI (ab2792; Abcam, 1:200), phospho-Tbk1 (5483;

(11) Acquisition of cellular properties during alveolar formation requires differential activity and distribution of mitochondriaeLifeApril 6, 2022Zhang K, Yao E, Chen B, Chuang E, Wong J, Seed RI, Nishimura SL, Wolters PJ, Chuang PT.RRID:AB_11182588,WB (1:2000) Antibody,Anti-Rat TGN38 (sheep polyclonal),Bio-Rad Laboratories,Cat# AHP499G; RRID:AB_2203272,IF (1:100) Antibody,"Anti-acetylated α-tubulin, clone 6-11B-1 (mouse monoclonal)",MilliporeSigma,Cat#

(12) Specific association of TBK1 with the trans-Golgi network following STING stimulationCell Structure and FunctionMarch 8, 2022Haruka Kemmoku, Yoshihiko Kuchitsu, Kojiro Mukai, Tomohiko TaguchiGoat Anti-Mouse IgG (H+L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G, dilution 1:200) (Bio-Rad); mouse anti-GM130 (610823, dilution 1:2,000) (BD Biosciences); rabbit anti-mCherry

(13) The activity of disease-causative STING variants can be suppressed by wild-type STING through heterocomplex formationFrontiers in cell and developmental biologyJanuary 1, 2022Shindo R, Kuchitsu Y, Mukai K, Taguchi T.eBioscience); rabbit anti-phospho-STING (D1C4T, dilution 1:400; Cell Signaling Technology); sheep anti-TGN38 (AHP499G, dilution 1:200; BioRad); rabbit-phospho-STING (D8F4W dilution 1:1000; Cell Signaling Technology); rabbit

(14) Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytesBasic research in cardiologyOctober 28, 2021Bogdanov V, Soltisz AM, Moise N, Sakuta G, Orengo BH, Janssen PML, Weinberg SH, Davis JP, Veeraraghavan R, Györke S.(Novus Biologicals, NBP2-34,842 and ThermoFisher Scientific, PA1-069A) [22, 60], TGN38 (Bio-Rad Laboratories, AHP499G and ThermoFisher Scientific, PA1-84,496) [13, 55, 58], and GM130 (BD, 558,712 and 610,823) [37, 45].

(15) Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axonsCell reportsApril 27, 2021Lie PPY, Yang DS, Stavrides P, Goulbourne CN, Zheng P, Mohan PS, Cataldo AM, Nixon RA.et al., 1990",N/A Rabbit anti-mouse CTSD,"Cataldo et al., 2004",N/A Sheep anti-TGN38,Bio-rad,Cat# AHP499G; RRID: AB_2203272 Rabbit anti-CTSD,Scripps Laboratories,Cat# RC245 Goat anti-CTSB,Neuromics,Cat# GT15047;

(16) The Parkinson's Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi NetworkCell reportsMay 5, 2020Beilina A, Bonet-Ponce L, Kumaran R, Kordich JJ, Ishida M, Mamais A, Kaganovich A, Saez-Atienzar S, Gershlick DC, Roosen DA, et al.(human specific),Biorad,cat # AHP500G; RRID:AB_323104 sheep anti-TGN38 (mouse specific),Biorad,cat # AHP499G; RRID:AB_2203272 rabbit anti-VPS35,GeneTex,cat # GTX108058; RRID:AB_1241448 mouse anti-ciM6PR,Abcam,"clone

(17) Golgi localized β1-adrenergic receptors stimulate Golgi PI4P hydrolysis by PLCε to regulate cardiac hypertrophyeLifeAugust 21, 2019Nash CA, Wei W, Irannejad R, Smrcka AV.cardiac myocytes.Mouse neonatal cardiac myocytes were stained with anti-β1AR (abcam ab3442-1:100) and anti-TGN38 (Biorad AHP499G-1:1000) using the saponin permeabilization protocol described in the Materials and methods. Scale bar

(18) Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transitionBoneMarch 1, 2016Yamamoto H, Ramos-Molina B, Lick AN, Prideaux M, Albornoz V, Bonewald L, Lindberg I.

(19) Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231PloS oneJanuary 1, 2016Tenorio MJ, Ross BH, Luchsinger C, Rivera-Dictter A, Arriagada C, Acuña D, Aguilar M, Cavieres V, Burgos PV, Ehrenfeld P, et al.GOLPH3 (Abcam; cat # ab98023), Syntaxin 16 (Synaptic Systems; cat # 110162), TGN38 (AbD Serotec; cat # AHP499G) and TGN46 (AbD Serotec; cat # AHP500G). The following fluorochrome-conjugated antibodies were from

(20) Identification of Quinolines that Inhibit Melanogenesis by Altering Tyrosinase Family TraffickingMolecular PharmacologyDecember 1, 2008Li Ni-Komatsu, ChunXiang Tong, Guangming Chen, Nelya Brindzei, Seth J. Orlow

Write a Review

(1)

7/17/2023Reliable TGN38 Antibody From Bio-Rad Evelyn HernandezUsed for probing for a Golgi apparatus protein for fractionation experiments. The antibody is for rats but it works well for mice too. I'd rate it a 4/5 because it is a suitable antibody but not ... Read Review

SHEEP ANTI RAT TGN38

SHEEP ANTI RAT TGN38

SHEEP ANTI RAT TGN38

SHEEP ANTI RAT TGN38

SHEEP ANTI RAT TGN38 from Bio-Rad (Formerly AbD Serotec)

Questions and Answers

Bio-Rad

Citations (20)

Reviews

SHEEP ANTI RAT TGN38 from Bio-Rad (Formerly AbD Serotec)

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map