Fig 1: Importance and role of AR-SV signaling in LIHC primary samples and HCC cell lines. (A) AR-SV expression of patients in the LIHC TCGA cohort (n = 372), broken down into four quartiles. Statistical significance was evaluated using the Mann–Whitney test. *, p < 0.05 for Q1 vs. Q4. (B) (Top) HCCLM3 and SNU475 cells were transfected with either a non-specific siRNA control or an siRNA targeting both AR-FL and AR-SVs. The AR status of siAR-transfected cells was confirmed by Western blot with anti-AR-NT (CS#5153, Cell Signaling) and GAPDH (CS#5174, Cell Signaling). RNA sequencing revealed 8479 differentially expressed genes in the HCCLM3 line and 8152 differentially expressed genes in the SNU475 line, with an intersection of 3701 genes. (Lower Left) Band intensities of HCCLM3 WT and AR KD Western blots. AR-SV #1 indicates the top AR-SV band and AR-SV #2 indicates the bottom AR-SV band. (Lower Right) Band intensities of SNU475 WT and ARKD Western blots. (C) GSEA of HCCLM3 and SNU475 ranked gene lists featuring intersecting up- and downregulated hallmark gene sets. FDR < 25%. (D) Highest-ranking up- and downregulated intersecting hallmark gene sets from GSEA on HCC cell lines. The uncropped bolts are shown in Supplementary Materials.