Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb 5546T from Cell Signaling Technology

Product Specs
ItemUbiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $174.00 Inquire
Catalog Number5546T
Quantity20 µl
ApplicationsW, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R, Mk
TargetH2B (Lys120) ubiquitinate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH2B clustered histone 3
NCBI Gene AliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight23

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) MPND Loss Epigenetically Activates TGFβ/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non–Small Cell Lung CancerCancer ResMay 26, 2026Jianfeng Yu, Jieru Zhang, Jun Zhang, Zhenqi Bai, Yunqing Zhang, Zi’an Liu, Lifan Su, Ye Yang, Yixin Lian, Wei Gu, et al.Cell Signaling Technology, RRID: AB_10891618), rabbit mAb against ubiquityl-histone H2B (Lys120; D11, 5546S, Cell Signaling Technology, RRID: AB_10693452), rabbit mAb against SMAD2 (D43B4, 5339S, Cell Signaling Technology

(2) PAF1C restores transcription after DNA damage independently of promoting histone mark depositionEMBO ReportsApril 8, 2026Janne J M van Schie, Bram A F J de Groot, Diana van den Heuvel, Martijn S Luijsterburgbath. ubH2B ChIP was performed using ~28 µg of chromatin with 3 µl of ubH2B antibody (Cell Signaling; #5546S) by overnight incubation at 4 °C. Protein-chromatin-pull down followed with a 1:1 mix of protein A and

(3) A cell cycle-dependent transition of acetylation to phosphorylation regulates timely centrosome maturationNat CommunMarch 20, 2026Jimin Li, Jianqiang Liang, Guifang Chen, Xuejie Wang, Yanyan Wang, Jingfei Zhan, Yunjing Guo, Jiadong Wang, Xuefeng Chen1:10000 for WB); anti-GAPDH (ABclonal, AC002, 1:5000 for WB); anti-H2Bub (Cell Signaling Technology, 5546, 1:1000 for WB); anti-RNF40 (Abcam, ab26082, 1:1000 for WB, 1:100 for IF); anti-α-tubulin (Proteintech,

(4) Structural insights into the Bre1–Lge1 and RNF20/RNF40–WAC interactions critical for H2B ubiquitinationNucleic Acids ResearchJanuary 14, 2026Meng Shi, Xuejie Wang, Hang Zhang, Yajiao Wen, Qianqian Liu, Pu Chen, Xuefeng Chen, Song Xianganti-FLAG (14793S, Cell Signaling Technology, RRID: AB_2 572 291, 1:200 diluted) or anti-H2BUb1 antibodies (5546, Cell Signaling Technology, RRID: AB_10 693 452, 1:200 diluted). The purified DNA was analyzed with

(5) LDOC1 connects histone H2B monoubiquitination to tumor cell plasticity in non-small cell lung cancerCell Communication and SignalingJanuary 3, 2026Hsien-Neng Huang, Pin-Feng Hung, Yi-Ta Tsai, En-Ting Liu, Tai-Lung Cha, Ya-Pin Chen, Wen-Tsan Weng, Chiao-Yin Sun, Wei-Hsuan Yu, Hau-Lun Cheng, et al.(Abcam, ab1790; WB, IP, IF); anti-H2Bub1 (ubiquityl-Histone H2B [Lys120], Cell Signaling Technology, #5546; WB, IF, ChIP); anti-GAPDH (GeneTex, GTX100118) and anti-THAP12 (Bethyl, A300-586 A; WB, IF, IP); anti-PSMA1

(6) Spt7 Deletion Reveals Vulnerabilities in Cryptococcus neoformans Stress Adaptation and VirulenceMicroorganismsJanuary 1, 2026Chendi Katherine Yu, Christina J. Stephenson, Benjamin L. Schulz, James A. Fraserused for detection of histone and histone modification marks: H2B (#12364), H3 (#4499); H2BK120ub (#5546), H3K9ac (#9649), H3K14ac (#7627), H3K18ac (#13998), H4K8ac (#2594), H4K12ac (#13944), H4K16ac (#13534)

(7) Distinct characteristics of VEXAS-causative UBA1 M41 and recurrent functional non-M41 mutationsLeukemiaDecember 1, 2025Sakuma M, Wang AK, Magaziner SJ, Keane SP, Meggendorfer M, Kern W, Haferlach C, Haferlach T, Beck DB, Walter W.ubiquitylated-H2A (Cell Signaling, 8240), H2A (Cell Signaling, 12349), ubiquitylated-H2B (Cell Signaling, 5546S), H2B (Cell Signaling, 12364), and β-actin (Cell Signaling, 4970) were used at a concentration of 1:1000

(8) H2BK120ub and its reader RNF169 sequentially regulate replication fork remodeling and stabilityThe EMBO journalNovember 1, 2025Duzanic FD, Mohana-Natarajan V, Fisicaro S, Bakker C, Aouami M, Amaral G, Lopes M, Taneja N, Penengo L.H3,Abcam,RRID:AB_302613 Rabbit anti-Histone H2B,Abcam,RRID:AB_302612 Rabbit anti-H2BK120ub,Cell Signaling,5546S Mouse anti-H3K9me3,Abcam,ab317790 Rabbit anti-Lamin A,Sigma-Aldrich,RRID:AB_532254 Rabbit anti-MCM7,Cell

(9) Endothelial RNF20 suppresses endothelial-to-mesenchymal transition and safeguards physiological angiocrine signaling to prevent congenital heart diseaseNature communicationsOctober 27, 2025Dou Y, Tetik-Elsherbiny N, Gao R, Ren Y, Chen YW, Merbecks M, Setya A, Lityagina O, Wang Y, Chichelnitskiy E, et al.anti-phospho-Histone H3 (Ser10) (1:200, Cell Signalling #9701S), anti-Ubiquityl-Histone H2B (Lys120) (1:200, Cell Signaling, 5546). Incubation with primary antibody was performed overnight at 4 °C in a humidified chamber. After three

(10) Paf1C regulates the Neurospora circadian clock by promoting the transcription elongation of frequencyPLOS GeneticsOctober 23, 2025Mengmeng Zhou, Yunpeng Zhao, Yubo He, Zeyu Duan, Xiao Liu, Qun Heanti-RTF-1(1:20000), rabbit anti-CTR-9 (1:5000), rabbit anti-PAF-1 (1:10000), rabbit anti-H2Bub (Cell signaling; 5546S; 1:2000) and rabbit anti-H2B (abcam; ab1790; 1:2000). RNA was extracted and analyzed by Northern blots

(11) The Glc7/PP1 phosphatase triggers Paf1C dissociation from RNA polymerase II to enable transcription terminationbioRxivSeptember 11, 2025Sanchirmaa Namjilsuren, Karen M. Arndt1:3000), anti-phospho-Spt5 (gift from Steven Hahn, Liu et al.,12 1:340), anti-H2BK123ub (Cell Signaling, 5546, 1:1000), anti-H3K4me1 (Millipore, 07-436, 1:2000), anti-H3K4me3 (Active Motif, 39159, 1:2000) and anti-G6PDH

(12) Genome-wide profiling and functional study of short N-terminal H2B variants in ArabidopsisJournal of Advanced ResearchSeptember 1, 2025Peng Yao, Qin He, Ying Wang, Danyang Sun, Xiangsong Chen, Li Lu(Cell Signaling Technology, 3724), anti-H3K9ac antibody (ABclonal, A7225) or anti-H2Bub (Cell Signaling Technology, 5546) overnight at 4℃. Pre-washed Protein A/G beads (SMART-Lifesciences, SA032005) were added and incubated

(13) RNF20/RNF40 supports the aggressive behavior in cervical cancer by regulating a peroxisome-based anti-ferroptotic mechanismCell Commun SignalJuly 1, 2025Shaishavi Jansari, Anna Blandau, Evangelos Prokakis, Daniela Grimm, Anja Maria Naßl, Stefan Küffer, Wiebke Möbius, Christian Dullin, Fernanda Ramos-Gomes, Leona Schüürhuis, et al.described previously [43] 72 h after siRNA-mediated transfection using antibodies against H2Bub1 (Cat. No. 5546 S, Cell Signaling Technology) followed by RT-qPCR. Cellular ROS measurement HeLa cells (12-well plates)

(14) RNF20/RNF40 supports the aggressive behavior in cervical cancer by regulating a peroxisome-based anti-ferroptotic mechanismCell Communication and SignalingJuly 1, 2025Shaishavi Jansari, Anna Blandau, Evangelos Prokakis, Daniela Grimm, Anja Maria Naßl, Stefan Küffer, Wiebke Möbius, Christian Dullin, Fernanda Ramos-Gomes, Leona Schüürhuis, et al.described previously [43] 72 h after siRNA-mediated transfection using antibodies against H2Bub1 (Cat. No. 5546 S, Cell Signaling Technology) followed by RT-qPCR. Cellular ROS measurement HeLa cells (12-well plates)

(15) Adipocyte RNF20 Knockout Leads to Hyperinsulinemia via the H2Bub-H3K4me3-Slc2a4 AxisJ Cell Mol MedJune 16, 2025Ying Zhao, Xiaojuan Liang, Jiayu Tang, Chunwei Cao, Chunhuai Yang, Shulin Yang, Jianguo Zhao, Jinxiang Yuan, Meng Zhang, Yanfang Wangused in this study, including β‐Tubulin (CST, 2146), RNF20 (Proteintech, 21625–1‐1‐AP), H2Bub (CST, 5546), H3K4me3 (Abcam, ab8580), H3K79me3 (Abcam, ab177185), PPARγ (CST, 2443), C/EBPα (Abcam, ab40764), AKT

(16) RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1αNat CommunMay 28, 2025Hao Liu, Yongqin Tang, Anshu Singh, Joaquim Vong, Julio Cordero, Arthur Mathes, Rui Gao, Yanhan Jia, Boyan K. Garvalov, Till Acker, et al.Cell Signaling), 1 µg Pol II p-Ser5 (ab5131, Abcam), 1 µg Pol II p-Ser2 (13499 s, Cell Signaling), 1 µg H2Bub1 (5546S, Cell Signaling) or 1 µg H3K4me3 (ab8580, Abcam) antibodies overnight at 4 °C, followed by binding to

(17) B4 Raf‐like MAPKKK RAF24 regulates Arabidopsis thaliana flowering time through HISTONE MONO‐UBIQUITINATION 2New PhytologistMay 20, 2025Qiaomu Li, Le Wang, Lauren E. Grubb, Mohana Talasila, Maria Camila Rodriguez Gallo, Devang Mehta, Sabine Scandola, Richard Glen Uhrigmembranes were incubated overnight with anti‐H2B (1 : 2500, ab1790; Abcam), anti‐H2Bub1 (1 : 1000, #5546; Cell Signaling, Boston, MA, USA), anti‐GFP (1 : 2000, PABG1; ChromoTek), anti‐HA(1 : 1000, 26183; Invitrogen),

(18) Highly specific Immunoproteasome inhibitor M3258 induces proteotoxic stress and apoptosis in KMT2A::AFF1 driven acute lymphoblastic leukemiaSci RepMay 19, 2025Tyler W. Jenkins, Jacquelyn Elise Fitzgerald, Jieun Park, Addison M. Wilson, Kristy L. Berry, Keith S. Wong, Walid A. Houry, Irene Lee, Andrey V. Maksimenko, Peter R. Panizzi, et al.AB_2295301), rabbit monoclonal Ubiquityl-Histone H2B(K120) antibody (clone D11, Cell Signaling Cat#5546; RRID: AB_10693452), K48-linkage specific polyubiquitin rabbit monoclonal antibody (clone D9D5, Cell Signaling

(19) USP22 promotes the proliferation and Sorafenib resistance of hepatocellular carcinoma cells via its deubiquitinase activityClin Transl MedMay 7, 2025Xiaochen Wang, Yijie Su, Bei Lan, Xuanyuan Li, Bodi Zhang, Liang Zhang, Yingmei Wang, Chunze Zhang, Chenghao Xuan(A12830), anti‐TFRC (A5865) and anti‐β‐actin (AC038) from ABclonal Technology Co., Ltd.; anti‐H2BK120ub (5546), anti‐His (12698), anti‐Ki67 (9027) and anti‐GAPDH (2118) from Cell Signalling Technology Inc.; anti‐H2AK119ub

(20) Increasing H2B Monoubiquitination Improves the Transcriptome and Memory in the Aged HippocampuseNeuroApril 7, 2025Shannon Kincaid, Gueladouan Setenet, Natalie J. Preveza, Kaiser C. Arndt, Phillip Gwin, Yu Lin, Hehuang Xie, Timothy J. JaromeBradford assay (Bio-Rad). Antibodies Antibodies included monoubiquitinated histone H2B (1:1,000, #5546, Cell Signaling Technology) and total histone H3 (1:1,000, #1791, Abcam). Western blot Normalized

Be the First to Write a Review!

Frontiers in Epigenetics and Epigenomics

Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb from Cell Signaling Technology

Description

Cell Signaling Technology

Citations (159)

Reviews

Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map