Western blot was performed using Anti-SV2A Polyclonal Antibody (Product # PA5-52476) and a 80kDa band corresponding to SV2A was observed across all cell lines and tissue tested where there is heavy glycosylation in the brain tissues. Whole cell extracts (30 µg lysate) of U-2-OS (Lane 1), undifferentiated SH-SY5Y (Lane 2), differentiated SH-SY5Y (Lane 3), undifferentiated PC-12 (Lane 4), differentiated PC-12 (Lane 5), NIH-3T3 (Lane 6), Mouse brain (Lane 7) and Rat brain (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4ug/ml) and detected by Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).