Normal human peripheral blood lymphocytes were stimulated with Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975) overnight. Cells were fixed and stained intracellularly with Anti-Human CD3 PerCP-Cyanine5.5 (Product # 45-0036-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-82) (left) or Anti-Human Amphiregulin PE (Product # 12-5370) (right) using the Intracellular Fixation and Permeabilization Buffers set (Product # 88-8824). Viable cells the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.
Supplier Page from Thermo Fisher Scientific for Mouse IgG1 kappa PE Isotype Control