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Mouse IgG1 kappa APC Isotype Control
Mouse IgG1 kappa APC Isotype Control from Thermo Fisher Scientific
(2)
Write a review
Citations:
(38)
Supplier Page
Supplier Page from
Thermo Fisher Scientific for
Mouse IgG1 kappa APC Isotype Control
Thermo Fisher Scientific's
Mouse IgG1 kappa APC Isotype Control
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, and Other.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Mouse IgG1 kappa APC Isotype Control
Company
Thermo Fisher Scientific
Price
Supplier Page
Catalog Number
17-4714-82
Quantity
100 µg
Applications
Control, Flow Cytometry
Concentration
0.2 mg/mL
Conjugate/Tag
APC
Format
PBS with 0.09% sodium azide; pH 7.2
Purity
Affinity chromatography
Target
Mouse IgG1 kappa
Isotype
IgG1 kappa
Clone
P3.6.2.8.1
Host
Mouse
Storage Temperature
4° C, store in dark, DO NOT FREEZE!
Antibody Type
Isotype Control
Clonality
Monoclonal
Storage
4°C, store in dark, DO NOT FREEZE!
Add to Compare List
Thermo Fisher Scientific
Thermo Fisher Scientific
81 Wyman Street
Waltham
,
MA
02451
United States
Phone
: 800-678-5599
Fax: 800-331-2286
Company Profile
Website:
www.thermofisher.com
Citations (38)
(1)
LDB1 represses fetal hemoglobin expression by enhancing BCL11A transcription
Redox Biol
February 4, 2026
Si-Won Park, Chang-Yong Choi, In-Byung Park, Seok-Jin Kang, Hyeon Jeong Lee, Ji-In Kim, Joonbeom Bae, Dongho Geum, Yong-Pil Cheon, Taehoon Chun
(
Thermo Fisher Scientific
MHFH05
, dilution
1:100
) or its isotype control (
Thermo Fisher Scientific
17-4714-82
, dilution
1:200
). 2.3
Colony forming unit
(CFU)
assay
Isolated CMPs (5 × 104 cells/well), GMPs (2.5
(2)
Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation
Nature Communications
December 12, 2025
Rajini Srinivasan, Tao Sun, Azalia Sandles, Diana Wu, Liang Wang, Hetal Patel, Ryan Pabalate, Maram Bader, Amy Heidersbach, Clark Ho,
et al.
No.
17-0819-42
), mouse IgG1 kappa isotype control antibody APC (
Thermo Fisher Scientific
, Cat. No.
17-4714-82
),
SSEA-4 antibody
Fluorescein
(
R&D Systems
, Cat. No.
FAB1435F
),
CD81 antibody PE
(
BioLegend
, Cat. No.
(3)
Feeder-Free Expanded and Cryopreserved NK Cells Retain Antibody-Dependent Cell Cytotoxicity against HER2-Positive Tumors
Journal of Microbiology and Biotechnology
October 29, 2025
Injee Lee, Suwoo Kim, Chaeyeon Jin, KyuBum Kwack, Youngseok Baek
(NCAM),CMSSB,
eBioscience
,
17-0567-42
FTIC,P3.6.2.8.1,
eBioscience
,
11-4714-42
PE,P3.6.2.8.1,
eBioscience
,
12-4714-42
APC,P3.6.2.8.1,
eBioscience
,
17-4714-82
PC.5.5,P3.6.2.8.1,
eBioscience
,
45-4714-82
"
CD3
, eFluor 450",UCHT1,
eBioscience
,
48-0038-42
Antibodies
(4)
Integrin CD103 expression in naive CD8+ T cells promotes cytokine-driven acquisition of memory phenotype and effector function
Immunity
September 15, 2025
Can Li, Davinna L. Ligons, Dominic Lanasa, Hilary R. Keller, Megan A. Luckey, Brian Capaldo, Daoud Meerzaman, Joo-Young Park, Jung-Hyun Park
RTK2071),
BioLegend
,Cat#:
400418
; RRID:AB_389341
Mouse
IgG1κ Isotype control – APC (
clone
(P3.6.2.8.1)),
eBioscience
,Cat#:
17-4714-82
; RRID:AB_763649
Mouse
IgG1κ Isotype control – eFluor 660 (
clone
P3.6.2.8.1),
eBioscience
,Cat#:
50-4714-82
;
(5)
Decitabine-Driven Foetal Haemoglobin Induction in Townes Mice and Human Erythroblasts
EJHaem
August 4, 2025
Ariadna Carol Illa, Desmond Wai Loon Chin, Martha Clark, Jesper Petersen, Søren Skov, Andreas Glenthøj, Carsten Dan Ley
HBF‐1, #
MHFH05
,
Invitrogen
) and an APC‐conjugated
mouse IgG1 kappa isotype control
(
Clone
: P3.6.2.8.1, #
17‐4714‐82
,
eBioscience
, Invitrogen), both at 0.83 µg/mL.
Flow cytometry
data were analysed with NovoExpress software
(6)
Harnessing macrophage-drug conjugates for allogeneic cell-based therapy of solid tumors via the TRAIN mechanism
Nature communications
February 4, 2025
Taciak B, Bialasek M, Kubiak M, Marszalek I, Gorczak M, Osadchuk O, Kurpiel D, Strzemecki D, Barwik K, Skorzynski M,
et al
.
control antibody (
1:20
,
400214
,
BioLegend
), APC mouse IgG1 κ isotype control antibody (P3.6.2.8.1) (
1:20
,
17-4714-82
,
eBioscience
) for 1 h on ice in the dark. Afterward, cells were washed twice with ice-cold PBS (Biowest,
(7)
TYRP1 directed CAR T cells control tumor progression in preclinical melanoma models
Molecular Therapy: Oncology
September 19, 2024
Christopher S. Hackett, Daniel Hirschhorn, Meixian S. Tang, Terence J. Purdon, Yacine Marouf, Alessandra Piersigilli, Narasimhan P. Agaram, Cailian Liu, Sara E. Schad, Elisa de Stanchina,
et al.
SJ25C1 conjugated to PE (Cat#
12-0198-42
) and
mouse IgG1 kappa isotype control
conjugated to APC (Cat#
17-4714-82
) were purchased from
eBioscience
/Thermo Scientific (Waltham, MA) and used at a concentration of 5 μL
(8)
Heart‐on‐a‐Chip Model of Epicardial–Myocardial Interaction in Ischemia Reperfusion Injury
Advanced Healthcare Materials
August 1, 2024
Dawn Bannerman, Simon Pascual Gil de Gomez, Qinghua Wu, Ian Fernandes, Yimu Zhao, Karl T. Wagner, Sargol Okhovatian, Shira Landau, Naimeh Rafatian, David F. Bodenstein,
et al.
1:20
) in the AldeRed
assay
buffer for 30 minutes at 4°C. Unstained, isotype control (
Thermo Fisher
,
17–4714-82
,
1:20
), and DEAB
inhibitor
of AldeRed ALDH Detection
Assay
(Sigma-Aldrich, SCR150) were included. All
(9)
Turning anecdotal irradiation-induced anticancer immune responses into reproducible in situ cancer vaccines via disulfiram/copper-mediated enhanced immunogenic cell death of breast cancer cells
Cell Death & Disease
April 27, 2024
Wei Guo, Lin Jia, Ling Xie, Juliann G. Kiang, Yangyang Wang, Fengfei Sun, Zunwen Lin, Enwen Wang, Yida Zhang, Peigen Huang,
et al.
CD47 monoclonal antibody [B6H12] (
17-0479-42
) and APC-conjugated
mouse IgG1 kappa isotype control
(
17-4714-82
) were purchased from
ThermoFisher
(Waltham, MA, USA). PE-conjugated rat anti-mouse CD274 (B7-H1, PD-L1)
(10)
BCG immunotherapy promotes tumor-derived T-cell activation through the FLT3/FLT3LG pathway in bladder cancer
Journal of Cancer
January 1, 2024
Zhang W, Yu L, Chang Z, Xiong H.
PBS and resuspended in 200 μL of PBS. Mouse IgG1 kappa Isotype Control-APC (P3.6.2.8.1) (
eBioscience
,
17-4714-82
) was used as a isotype for
FLT3
staining
.
Flow cytometry
data were acquired using CytoFLEX LX (Beckman
(11)
Characteristics of multilineage-differentiating stress-enduring cell clusters in different culture conditions
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
November 1, 2023
Ning J, Cao YY, Zhang RZ, Li Y.
cat. no.
12‐4341‐81
;
Thermo Fisher Scientific
, Inc.) and a
CD105
isotype antibody (
1:100
; cat. no.
17‐4714‐82
;
Thermo Fisher Scientific
, Inc.) were used according to the recommended dosage. Those antibodies in
(12)
Platelet-derived extracellular vesicles promote endothelial dysfunction in sepsis by enhancing neutrophil extracellular traps
BMC immunology
August 9, 2023
Jiang M, Wu W, Xia Y, Wang X, Liang J.
APC-conjugated
CD61
(
17-0619-42
,
eBioscience
, San Diego, CA, USA) or the respective isotype control (
17-4714-82
,
eBioscience
, San Diego, CA, USA) for 30 min in the dark at room temperature. After that, the sample
(13)
Low T-cell reactivity to TDP-43 peptides in ALS
Frontiers in Immunology
July 21, 2023
Swetha Ramachandran, Veselin Grozdanov, Bianca Leins, Katharina Kandler, Simon Witzel, Medhanie Mulaw, Albert C. Ludolph, Jochen H. Weishaupt, Karin M. Danzer
BioLegend
,
400151
), Mouse IgG1, κ-PE (
1:100
,
BioLegend
,
400112
), Mouse IgG1, κ-APC (
1:100
,
Invitrogen
,
17-4714-82
), Mouse IgG2a, κ- PE/Cy7 (BD Biosciences,
1:20
,
400232
),Mouse IgG2a, κ-FITC(
1:20
,
BioLegend
,
400208
),
(14)
Evaluation of quantitative biomarkers of aging in human PBMCs
Front Aging
January 1, 2023
Owen BM, Phie J, Huynh J, Needham S, Fraser C.
kappa-APC (
Miltenyi
130-113-269
), REA IgG1 APC (
Miltenyi
130-113-446
),
IgG1
k APC (
Thermo scientific
17-4714-82
). FACS buffer was cooled on ice before use. The PBMCs were aliquoted at 100,000 cells per well of a
(15)
Anti-tumor effect of antibody drug conjugate ASP1235 targeting Fms-like tyrosine kinase 3 with venetoclax plus azacitidine in an acute myeloid leukemia xenograft mouse model
Oncotarget
December 20, 2022
Tsuzuki H, Kawase T, Nakazawa T, Mori M, Yoshida T.
400134
), APC conjugated mouse IgG1 κ isotype control antibody (
Thermo Fisher Scientific
, Catalog #
17-4714-82
, RRID: AB_763649). Dead cells were excluded by using
Zombie Violet Fixable Viability Kit
(
BioLegend
,
(16)
CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast Cancer
Cancers
November 23, 2021
Magagna I, Gourdin N, Kieffer Y, Licaj M, Mhaidly R, Andre P, Morel A, Vincent-Salomon A, Paturel C, Mechta-Grigoriou F.
IgG1 isotype (
1:100
,
Invitrogen
, #
46-4714-82
) and APC mouse IgG1 isotype control (
1:80
,
Invitrogen
, #
17-4714-82
).
T cells
were washed with PBS+ and centrifuged at 1800 rpm for 10 min at 4 °C. FOXP3 staining buffer
(17)
A road map from single-cell transcriptome to patient classification for the immune response to trauma
JCI insight
January 25, 2021
Chen T, Delano MJ, Chen K, Sperry JL, Namas RA, Lamparello AJ, Deng M, Conroy J, Moldawer LL, Efron PA,
et al
.
17-9852-80
), and anti-IgG1 κ isotype control APC (P3.6.2.8.1) (eBioscience,
Thermo Fisher Scientific
, catalog
17-4714-82
). Computation of PC-associated genes and PC functional annotation. For the PC of interest, we computed
(18)
GP IIb/IIIa-Mediated Platelet Activation and Its Modulation of the Immune Response of Monocytes Against
Candida albicans
Frontiers in cellular and infection microbiology
January 1, 2021
Zheng L, Duan Z, Tang D, He Y, Chen X, Chen Q, Li M.
clone
Psel
.K02.3) or
1:150
-diluted immunoglobulin isotype control of P-selectin-APC (
Thermo Fisher
,
17-4714-82
,
clone
P3.6.2.8.1), followed by
incubation
for 30 min at room temperature. After washing, the samples
(19)
Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis
Experimental and therapeutic medicine
December 1, 2020
Wang Q, Sun Q, Chen Q, Li H, Liu D.
used as negative controls: APC‑conjugated
Mouse IgG1 kappa Isotype Control
(2.5 or 0.6 µg/ml; cat. no.
17‑4714‑82
), FITC‑conjugated Mouse IgG1 Isotype Control (10 µg/ml; cat. no. MA1‑10413), PE‑Cyanine 5‑conjugated
(20)
Targeting QKI-7 in vivo restores endothelial cell function in diabetes
Nature communications
July 30, 2020
Yang C, Eleftheriadou M, Kelaini S, Morrison T, González MV, Caines R, Edwards N, Yacoub A, Edgar K, Moez A,
et al
.
(
Thermo Fisher Scientific
17-1449-42
,
1:20
) and Mouse IgG1 kappa Isotype Control-APC (
Thermo Fisher Scientific
17-4714-82
,
1:800
) were used.
Luciferase reporter assay
For luciferase reporter
assays
, 4 × 104/well of iPS-ECs
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Reviews
Write a Review
(1)
11/28/2022
Great Isotype Control
Dimi Comfu
Our research is heavily relied on cell-based assays. We often need to characterize cell lines or primary cells, using FACs, which requires the usage of isotype controls.
(1)
10/15/2019
A Good Isotype Antibody for Assessing Non-Specific Antibody Binding on Peripheral B Cells by Flow Cytometry
Chris Corkum,
This antibody is recommended for use as an isotype control in combination with appropriate test antibodies for flow cytometry experiments involving purified B cells from peripheral blood mononuclear ...
Read Review
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Mouse IgG1 kappa APC Isotype Control from Thermo Fisher Scientific
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