Fig 1: Localization of mitochondrial (MRPS27) and cytoplasmic (RPS6 and RPL3) ribosomal proteins in human spermatozoa. Purified human spermatozoa were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton-X-100 and stained with anti-MRPS27, -RPS6, or -RPL3 antibodies. Red: Ribosomal proteins, blue: DAPI staining of the nucleus, green: PSA-FITC staining of the acrosome. Scale bar: 10 μm. Representative results of N = 3 experiments. BH: base of the head, MP: Midpiece, N: Neck.