PE anti-mouse H-2Kb/H-2Db 114607 from BioLegend

Product Specs
ItemPE anti-mouse H-2Kb/H-2Db
CompanyBioLegend
Price $129.00Supplier Page View Company Product Page
Catalog Number114607
ApplicationsFC
Clone28-8-6
HostMouse
ReactivityMouse
Conjugate/TagPE
IsotypeMouse (C3H) IgG2a, κ
Quantity50UG
Antibody TypePrimary
NCBI Gene AliasesK-f, H2-K1, H2-D1, H-2D, H-2K, H-2K(d), H2-K, H2-D
NCBI Full Gene Namehistocompatibility 2, K1, K region, histocompatibility 2, D region locus 1
Concentration0.2000mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenC3H.SW mouse splenocytes
ClonalityMonoclonal
TargetH-2Kb/H-2Db

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Therapeutic DC vaccination against the vascular antigen DLK1 effectively treats primary triple-negative breast cancer and prevents metastasisnpj VaccinesJune 23, 2026Elizabeth A. Daugherity, Caryn Lawrence, Izuchukwu F. Okpalanwaka, Tyler Mouw, Dauod Arif, Duke Appiah, Walter J. Storkus, Devin B. LoweBioLegend). DCs collected from C57BL/6 mice where further stained with PE-H2-Kb/Db (clone 28-8-6, cat# 114607, BioLegend) while PE-H2-Kd (clone SF1-1.1.1, cat# 5957-80, eBiosciences) and PE-H-2Dd (clone 34-2-12,

(2) High-titer modular retroviral vectors enabled by an antisense cassette design preventing dsRNA formation during virus productionMolecular Therapy AdvancesJune 11, 2026Romain Vuillefroy de Silly, Patrick Reichenbach, Melita Irvinganalyze viable cells. For surface marker expression, cells were stained with anti- H-2Kb/H-2Db-PE (114607), CD44-APC (103011), CD45RB-APC (103319), CD54-APC (116119), and CD90.1/Thy-1.1-BV421 (202529) antibodies

(3) DGAT1 Inhibition Induces Ferroptosis and Enhances Cancer Immunotherapy EfficacyCancer ResMarch 11, 2026Dong Pan, Meng Jiao, Yanyan Zhu, Mengjie Hu, Fang Li, Jinming Yu, Chuan-Yuan Liantibody (BioLegend, cat. #153612, RRID:AB_2894673) or PE anti-mouse H2Kb/H2Db antibody (BioLegend, cat. #114607, RRID:AB_313598) on ice for 20 minutes in the dark. After staining, the cells were washed twice with

(4) Gamma-retrovirus design for efficacious microRNA-mediated gene knock-down and protein co-expression without compromising titersNAR Molecular MedicineAugust 12, 2025Romain Vuillefroy de Silly, Bili Seijo, Jimmy Maillard, Patrick Reichenbach, Melita Irvingto analyze viable cells. For surface marker expression, cells were stained with anti-H-2Kb/H-2Db-PE (114607), CD8α-BV421 (100738), CD25‐APC (102012), CD44‐APC (103011), CD45RB-APC (103319), CD54-APC (116119),

(5) Different tumour-resident memory T-cell subsets regulate responses to anti-PD-1 and anti-CTLA-4 cancer immunotherapiesNature communicationsJuly 1, 2025Damei I, Caidi A, Auclin E, Adam J, Mella S, Hasan M, Tartour E, Robert C, Corgnac S, Mami-Chouaib F.(clone TW7-20B9, 141305, 1/100), CD274 (PD-L1, clone 10 F.9G2, 124313, 1/100), H2kb/H2Db (clone 28-8-6, 114607, 1/100) and I-A/I-E (clone M5/114.15.2, 107607, 1/100), were supplied by Biolegend. Anti-CD49a (clone

(6) EZH2 loss during metabolic stress drives restoration of MHC Class I machinery in melanomaiScienceJune 20, 2025Jacob L. Edmondson, Megan R. Reed, Daniel Fil, Billie Heflin, Alyson McKinnon, Michael A. Bauer, Lauren C. Morehead, Nathan L. Avaritt, Mary Phillips, Sean D. Taverna, et al.determined on a LSRFortessa flow cytometer (BD Biosciences) by staining for murine pan-MHC-I (Biolegend #114608), murine PDL1 (Biolegend #124312), human MHC-I (Biolegend #311406) and human PDL1 (Biolegend #393610).

(7) Zonated Copper‐Driven Breast Cancer Progression Countered by a Copper‐Depleting Nanoagent for Immune and Metabolic ReprogrammingAdvanced ScienceApril 24, 2025Lin Chen, Saibo Ma, Hao Wu, Lingna Zheng, Yunpeng Yi, Guangnian Liu, Baoyi Li, Jiayi Sun, Yang Du, Bing Wang, et al.(101222), anti‐mouse CD80‐PE‐Cy7 (104733), anti‐mouse CD86‐APC (105113), and anti‐mouse MHC Class I‐PE (114608) antibodies were purchased from BioLegend Inc. (San Diego, CA, USA). CCS antibody (sc‐55561) was obtained

(8) Targeting copper homeostasis: Akkermansia-derived OMVs co-deliver Atox1 siRNA and elesclomol for cancer therapyActa Pharmaceutica Sinica BMarch 10, 2025Muhammad Hamza, Shuai Wang, Hao Wu, Jiayi Sun, Yang Du, Chuting Zeng, Yike Liu, Kun Li, Xili Zhu, Huiying Liu, et al.(101222), anti-mouse CD80-PE-Cy7 (104733), anti-mouse CD86-APC (105113), and anti-mouse MHC Class I-PE (114608) and APC anti-mouse IFN-γ (505810) were purchased from BioLegend (San Diego, USA). 2.2 Methods 2.2.1

(9) Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in MelanomaCancer DiscoveryJanuary 28, 2025Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal, Osnat Bartok, Ronen Levy, Marie J. Zemanek, Chen Weller, Ella Herzog, Winnie Yao, et al.Flow Cytometry All SCCs were stained separately for assessment of the expression of MHC-I (BioLegend, Cat. #114607, RRID: AB_313598) and MHC-II (BioLegend, Cat. #116405, RRID: AB_313724) using flow cytometry. Cells

(10) Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in MelanomaCancer DiscovNovember 20, 2024Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal, Osnat Bartok, Ronen Levy, Marie J. Zemanek, Chen Weller, Ella Herzog, Winnie Yao, et al.Flow Cytometry All SCCs were stained separately for assessment of the expression of MHC-I (BioLegend, Cat. #114607, RRID: AB_313598) and MHC-II (BioLegend, Cat. #116405, RRID: AB_313724) using flow cytometry. Cells

(11) Lymph node-targeted STING agonist nanovaccine against chronic HBV infectionCellular and molecular life sciences : CMLSAugust 28, 2024Hu Y, Yang A, Li H, Zhao R, Bao C, Yu Y, Wang Y, Wang Z, Zhuo L, Han Q, et al.(MHC-II) (Cat:# 107645), FITC anti- mouse CD3e (Cat:# 100204), PE anti-mouse H-2Kb/H-2Db (MHC-I) (Cat:# 114608), PE/DazzleTM594 anti-mouse CD8α (Cat:# 100762), PE/DazzleTM594 anti-mouse IL-2 (Cat:# 503840), PerCP/Cyanine5.5 anti-mouse CD11a

(12) Resveratrol induces major histocompatibility complex class I antigen presentation in a STING-dependent and independent manner in melanomaMolecular ImmunologyNovember 1, 2023Lauren C. Morehead, Brian Koss, Daniel Fil, Billie Heflin, Sarita Garg, Katherine F. Wallis, Alan J. Tackett, Isabelle R. MiousseREAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies H-2KB/DB,Biolegend,"Cat# 114607, RRID:AB_313598" "HLA-A,B,C",Biolegend,"Cat# 311405, RRID:AB_314874" PD-L1,Biolegend,"Cat# 124311,

(13) Targeting Microglial Metabolic Rewiring Synergizes with Immune-Checkpoint Blockade Therapy for GlioblastomaCancer discoveryApril 3, 2023Ye Z, Ai X, Yang K, Yang Z, Fei F, Liao X, Qiu Z, Gimple RC, Yuan H, Huang H, et al.141706), anti-CD8a FITC (Biolegend, 100706), anti-CD4 APC (Biolegend, 100516), anti-MHCI PE (Biolegend, 114607), anti-Foxp3 PE (BD biosciences, 563101), anti-IFN-γ PE (BD biosciences, 554412), anti-TNF-α PE/Cyanine7

(14) Bacterial outer membrane vesicle based versatile nanosystem boosts the efferocytosis blockade triggered tumor-specific immunityNature communicationsMarch 25, 2023Zhuang WR, Wang Y, Nie W, Lei Y, Liang C, He J, Zuo L, Huang LL, Xie HY.

(15) Meningeal macrophages protect against viral neuroinfectionImmunityNovember 8, 2022Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, et al.C068C2),BioLegend,"Cat# 141720, RRID:AB_2562248" PE mouse (C3H) anti-mouse H-2Kb/H-2Db (clone 28–8–6,BioLegend,"Cat# 114607, RRID:AB_313598" Alexa Fluor 700 rat monoclonal anti-mouse CD8a (clone SK1),BioLegend,"Cat# 100730,

(16) Mutant IDH Inhibits IFNγ-TET2 Signaling to Promote Immunoevasion and Tumor Maintenance in CholangiocarcinomaCancer discoveryMarch 1, 2022Wu MJ, Shi L, Dubrot J, Merritt J, Vijay V, Wei TY, Kessler E, Olander KE, Adil R, Pankaj A, et al.TNF-α (BioLegend, Cat# 506321; Clone: MP6-XT22; RRID:AB_961435; 1:100), H-2Kb/H-2Db (BioLegend Cat# 114608, RRID:AB_313599). Generation of Tet2 or Ifngr1 Knockout Cell Lines Using SCAR Technology Tet2- or

(17) Impaired CXCL12 signaling contributes to resistance of pancreatic cancer subpopulations to T cell-mediated cytotoxicityOncoimmunologyJanuary 1, 2022Lin YN, Schmidt MO, Sharif GM, Vietsch EE, Kiliti AJ, Barefoot ME, Riegel AT, Wellstein A.cells were labeled with fluorescence-conjugated PD-L1 (# 124315 – dilution 1.5/200), H-2Kb/H-2 Db (# 114607 – dilution 1/200) and CXCR4 (# 146505 – dilution 3/200) antibodies (BioLegend) and analyzed by FACS

(18) Tumor evolution selectively inactivates the core microRNA machinery for immune evasionNature communicationsDecember 1, 2021Song TY, Long M, Zhao HX, Zou MW, Fan HJ, Liu Y, Geng CL, Song MF, Liu YF, Chen JY, et al.BV605 anti-CD44 (563058), BV605 anti-CD25 (563061) purchased from BD Biosciences; PE anti-H2Kb/H2Db (114607), APC anti-H2Kb bound to SIINFEKL (141605), FITC anti-Lag-3 (369308), FITC anti-CD69 (104506) and AF594

(19) Enhancing proteasomal processing improves survival for a peptide vaccine used to treat glioblastomaScience Translational MedicineJune 16, 2021Mario Fidanza, Puja Gupta, Anin Sayana, Varun Shanker, Svenja-Maria Pahlke, Brandon Vu, Fanny Krantz, Aruna Azameera, Nicollette Wong, Navya Anne, et al.isothiocyanate, phycoerythrin (PE) anti-human HLA-B7 (BioLegend, 372403), or PE anti-house H-2Kb (BioLegend, 114607) specific monoclonal antibodies before flow cytometric analysis on the NovoCyte 2000 (ACEA Biosciences

(20) Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technologyNature communicationsApril 6, 2021Cheng K, Zhao R, Li Y, Qi Y, Wang Y, Zhang Y, Qin H, Qin Y, Chen L, Li C, et al.

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PE anti-mouse H-2Kb/H-2Db from BioLegend

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PE anti-mouse H-2Kb/H-2Db from BioLegend

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