Fig 1: Detection of ILC2 in the ear skin of naïve C57BL/6 and BALB/c mice. Flow cytometry of ear skin cells from naïve 4get-C57BL/6 (A) and 4get-BALB/c mice (B). Lymphomononuclear cells were isolated from ear dermis and epidermis by enzymatic and mechanical dissociation. Single cells were pre-gated on viability and CD45 expression; eosinophils staining positively for SiglecF were excluded. ILC2 were defined as lineage marker-negative, CD90.2+ cells. For further characterization, the surface markers CD127, Sca-1, ICOS (CD278), PD1 and ST2 were used. Data are representative of 3 to 4 experiments (A,B) or 2 experiments (C) with 3 to 5 mice pooled per group. The lineage marker-mix included antibodies directed against CD2, CD3, CD5, CD11b, CD11c, CD19, FcεR1a, Ly6G, NKp46 and Ter119. The numbers within the histograms indicate the mean fluorescence intensity of the respective surface markers (A–C).