PE/Cyanine7 anti-mouse CD4 100528 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-mouse CD4
CompanyBioLegend
Price $198.00Supplier Page View Company Product Page
Catalog Number100528
NCBI Gene AliasesL3T4, Ly-4
Conjugate/TagPE/Cyanine7
IsotypeRat IgG2a, kappa
Quantity100 µg
CloneRM4-5
HostRat
ReactivityMouse
ApplicationsFC - Quality tested
NCBI Full Gene NameCD4 antigen
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
Antibody TypePrimary
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Loss of Akt3 enhances antiviral immunity while disrupting corneal homeostasis during ocular HSV-1 infectionFront ImmunolAugust 12, 2026Rashmi Kadam, Chandrashekhar D. Patil, Hemant Borase, Rakesh Rahangdale, Ipsita Volety, Soromidayo Akinsiku, Deepak Shukla(clone 17A2),BioLegend,Cat#100236 RRID: AB_2561456 Anti-mouse CD4-PE-CY7 (clone RM4-5),BioLegend,Cat#100528; RRID: AB_312729 Anti-mouse CD8a-PerCP/Cy5.5 (clone 53-6.7),BioLegend,Cat#100734; RRID: AB_2075238 Anti-mouse

(2) A Quartet of Native Orai Channel Isoforms Orchestrates Graded NFAT Activation and TranscriptionbioRxivJuly 13, 2026Ahmed Emam Abdelnaby, Yin-Hu Wang, J. Cory Benson, Isagani Perez, Mark Shen, Maxwell McDermott, Miki Jishage, Amal T. Elhaw, Silvia Cruz-Rangel, Raphael Courjaret, et al.Orai1fl/flCd4Cre mice were first stained with either PE-Cyanine7 conjugated anti-CD4 (Biolegend, Cat. 100528) or APC-Cyanine7 conjugated anti-CD4 (Biolegend, Cat. 100414). Cells were then washed, combined, and

(3) Bcl11b dose-dependently regulates positive selection of CD8 T cells to the virtual memory fatebioRxivJuly 9, 2026Tom Sidwell, Ellen V Rothenbergclone 17A2, lot B365335), CD3-FITC (BioLegend 100204, clone 17A2, lot B415733), CD4-PEcy7 (BioLegend 100528, clone RM4–5, lot B391737), CD4-PE (BioLegend 100512, clone RM4–5, lot B335844), CD4-Biotin (BioLegend

(4) DCIR-mediated inhibitory regulation of TLR7-MyD88 axis prevents autoimmune neuroinflammationiScienceJune 19, 2026Atsushi Fujioka, Kenji Shimizu, Saki Nakayama, Dai Ueno, Yuki Chiba, Ichiro Nakashima, Juichi Fujimori, Yuko Shirota, Yoichiro Iwakura, Yasushi Muraki, et al.AlexaFluor700(CloneRA3-6B2),BioLegend,Cat#103231;RRID;AB_493716 Anti mouse CD4 PE/Cy7(CloneRM4-5),BioLegend,Cat#100527;RRID;AB_312728 Anti mouse CD8 APC(Clone53-6.7),BioLegend,Cat#100711;RRID;AB_312750 ,, Biological

(5) Neuronal responses to cytokines limit intestinal hypermotility and systemic effects of colonic inflammationiScienceJune 19, 2026Jisun Jung, Alec Wehmeier, Zili Xie, David Horton, Emilie V. Russler-Germain, Ellen Merrick Schill, Mark J. Miller, Jonathan R. Brestoff, Rodney Newberry, Brian S. Kim, et al.PB,Biolegend,Cat#104424 anti-mouse/human CD44 (IM7) PECy7,Biolegend,Cat#103030 anti-mouse CD4 (RM4-5) PECy7,Biolegend,Cat#100528 anti-mouse CD8a (53-6.7) APCCy7,Biolegend,Cat#100714 anti-mouse FOXP3 (FJK-16s) FITC,ThermoFisher,Cat#11-5773-82 anti-mouse

(6) EZH1/2 inhibition improves immunotherapy response through MHC Class II de-repression and neutrophil reprogrammingbioRxivJune 4, 2026Avery R. Childress, Dave-Preston Esoe, Xiulong Song, Christain M. Gosser, Yindan Lin, Daniel R. Plaugher, Tanner J. DuCote, Kassandra J. Naughton, Erika M. Skaggs, Harrison Yang, et al.(BioLegend #100308, RRID:AB_312673), PD-1-PerCP (BioLegend #135208, RRID:AB_2159184), CD4-PECy7 (BioLegend #100528, RRID:312729), CD45-APC (BioLegend #103112, RRID:AB_312977), Ly6G-PerCP (BioLegend #127616 RRID:AB_1877271),

(7) A recombinant IgG1 Fc-domain protein ameliorates inflammatory demyelinating peripheral neuropathyFrontiers in ImmunologyMay 27, 2026Husniye G. Otlu, Tong Gao, Greg P. Coffey, Jessica M. Bright, Alyssa M. A. Toda, Pamela B. Conley, Kazim Sheikh, Gang ZhangPE/Cyanine5 anti-mouse CD3,17A2,Biolegend,100274,1:50 PE anti-mouse CD39,Duha59,Biolegend,143804,1:50 PE/Cyanine7 anti-mouse CD4,RM4-5,Biolegend,100528,1:50 BV605 anti-mouse CD19,6D5,Biolegend,115539,1:50 Antibodies and reagents used in flow cytometry.

(8) SNAP-23 mediated vesicular trafficking in oligodendrocytes is necessary to maintain adult myelin integrity in miceNature communicationsMay 25, 2026Chow CH, Huang M, Regmi A, Rai J, Harada H, Eide S, Sun HS, Feng ZP, Monnier PP, Okamoto K, et al.(clone 30-F11, Biolegend, 103154), F4/80 (clone BM8, Biolegend, 123108), CD4 (clone RM4-5, Biolegend, 100528), CD8 (clone 53-6.7, Biolegend, 100750), CD45R/B220 (clone RA3-6B2, Biolegend, 103212), and CD19 (clone

(9) Label-free proteomics and single-cell mass cytometry analysis of 5 immune tissues from mice with artesunate administration reveal immunosuppression of artesunate via BST2 and MDSCsFundamental ResearchApril 12, 2026Rongjian Hong, Aiting Wang, Yiyang Li, Xianting Dingantibodies including PE-CD3 (Biolegend, 100307), APC-CD8a (Biolegend, 100711), PE-Cy7-CD4 (Biolegend, 100527), APC-Gr1 (Biolegend, 108411), FITC-CD11b (Biolegend, 101205), PE-CD45 (Biolegend, 103105) were diluted

(10) TGF-α/EGFR-mediated lymphatic metastasis reveals a repositionable therapeutic target in breast cancerNPJ Breast CancerApril 3, 2026Wenyang Shi, Yueyun Pan, Bhavik Rathod, Yuhan Wang, Zhi Wang, Jianyu Shen, Francesca Gatto, Mingzhi Liu, Yizhe Sun, Margareta Wilhelm, et al.markers including VioBright FITC-anti-CD8a (130-120-822, clone REA601, Miltenyi Biotec), PE-Cy7-anti-CD4 (100528, clone RM4-5, BioLegend), BV605-anti-CD25 (102035, clone PC61, BioLegend), and BV650-anti-CD3 (100229,

(11) A stromal PAI1-tPA axis orchestrates immunosuppression in pancreatic cancerSci AdvApril 3, 2026Tenzin Ngodup, Brynn Elson, Ashley M. Mello, Sean Hannifin, Miranda Liu, Yaqing Zhang, Jiaqi Shi, Yatrik M. Shah, Daniel A. Lawrence, Marina Pasca di Magliano, et al.BV421-conjugated anti-CD3 (145-2C11, 1:100, no. 100341, BioLegend), PE-Cy7–conjugated anti-CD4 (RM4-5, 1:200, no. 100527, BioLegend), AF700-conjugated anti-CD8a (53-6.7, 1:300, no. 100730, BioLegend), APC-Cy7–conjugated anti-CD19

(12) DNA delivered by lipid nanoparticles induces CD8+ T cell–dependent antitumor responses and enhances anti–PD-L1 therapyJCI InsightMarch 23, 2026Seoyun Yum, Alba Rodríguez-Garcia, Joan Castellsagué, Marta Giménez-Alejandre, Guillem Colell, Salut Colell, Teresa Lobo-Jarne, Mark A. LaRue, Michael A. Minnier, Mustafa N. Yazicioglu, et al.CD16/32 for blocking (101301, clone 93), CD45.2 (103127, clone 30-F11), CD3 (100217, clone 17A2), CD4 (100527, clone RM4-5), CD8 (100707, clone 53-6.7), NK1.1 (108709, clone PK136), CD69 (104527, clone H1.2F3),

(13) MyD88 Inhibition Ameliorates Diabetes-Induced Hepatic Inflammation and Gluconeogenesis Through Adipose IL-10 InductionInt J Mol SciMarch 23, 2026Yi-Cheng Li, Hsiao-Chi Lai, Pei-Hsuan Chen, Chia-Hua Tang, Lee-Wei Chenintracellular Foxp3 staining, cells were first stained with anti-mouse CD4 (BioLegend, San Diego, CA, USA, #100528), fixed with True-Nuclear™ Fix Buffer for 1 h, and incubated with PE-conjugated anti-mouse Foxp3 antibody

(14) Mesenchymal stem cells ameliorate Sjögren disease by suppressing B cells through the Pik3cb/Akt/mTOR pathwayFront ImmunolMarch 12, 2026Zhifang Wu, Chunning Wang, Linsha Ma, Dengsheng Xiaflow cytometry. Briefly, the cells were stained with the following antibodies: anti-mouse CD4-PE/Cy7 (100528; BioLegend, USA), anti-mouse CXCR5-PerCP/Cy5.5 (145508; BioLegend, USA), anti-mouse B220-FITC (103206;

(15) RNA binding protein Sam68 promotes germinal center reaction and IgG response through regulation of miR29Cell Mol Life SciMarch 7, 2026Moumita Datta, Valerio Renna, Manish Kumar, Palash Chandra Maity, Hassan JumaaBlue,11-26c.2a,405712,Biolegend Thymus/spleen T cells,Thy 1.2,PE,30-H12,105307,Biolegend Thymus/spleen T cells,CD4,PE Cy7,RM4-5,100528,Biolegend Thymus/spleen T cells,CD8a,BV785,53–6.7,100749,Biolegend GC analysis,CD19,BV421,6D5,115538,Biolegend GC

(16) Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarctionBioact MaterMarch 4, 2026Qinfeng Ding, Yilong Teng, Hong Zhu, Zhitong Jia, Shuo Xu, Bin Hu, Boyan Chen, Jiewen Dai, Jian Sun, Ping Nie, et al.Zombie NIR™ Fixable Viability Kit (423105, Biolegend), CD45-BV421 (103134, Biolegend), CD3-APC (100235, Biolegend), CD4-PE/Cy7 (100527, Biolegend), CD8-BV510 (100752, Biolegend), CD11b-PE/Cy7 (101215, Biolegend), Ly-6G-PE (127607), Ly-6C-BV510

(17) Integrated computational and experimental immunoengineering of adeno-associated virus capsid T cell epitopes in miceNature CommunicationsMarch 3, 2026Sojin Bing, Arya Eskandarian, Sean Smith, Abdul Mohin Sajib, Stephanee Warrington, Sima Saleh, Susana S Najera, Rebecca J. D’Esposito, Luis Santana-Quintero, Ronit Mazor(1:1000, clone 500A2, BioLegend, cat. 560771, RRID: AB_2564590), CD4 (1:1000, clone RM4-5, BioLegend, cat. 100528, RRID: AB_312729), CD8 (1:1000, clone 53-6.7, BioLegend, cat. 100706, RRID: AB_312745), and CD69 (1:1000,

(18) Inflammation in a severe model of dystrophic cardiomyopathy contains a high proportion of T cells that contribute to onset of pathologyAmerican Journal of Physiology-Heart and Circulatory PhysiologyFebruary 1, 2026Arden B. Piepho, Swathy Krishna, Sarah L. Sturgill, Jeovanna Lowe, Zoe Wolfe, Rachel M. Pomeranets, Sachi Walmiki, Rachel Rosenzweig, Zachary M. Howard, Mark T. Ziolo, et al.CD45 (Super Bright 600; Invitrogen, 63045180), CD3 (FITC; BioLegend, 100306), CD4 (PE-Cy7; BioLegend, 100528) and CD8 (BV650; BioLegend, 100742). The markers were used to identify leukocytes (CD45+), T cells (CD3+),

(19) Methionine Restriction Reduces Lung Cancer Progression and Increases Chemotherapy ResponsebioRxivJanuary 16, 2026Kassandra J. Naughton, Xiulong Song, Avery R. Childress, Erika M. Skaggs, Aria L. Byrd, Christian M. Gosser, Dave-Preston Esoe, Tanner J. DuCote, Daniel R. Plaugher, Alexsandr Lukyanchuk, et al.by flow cytometry with CD45-APC (BioLegend 103112), CD3e-PE (BioLegend 100308), CD4-PECy7 (BioLegend 100528) and CD8-FITC (BioLegend 100706). T-cells were plated in 96 well plates in media with methionine concentrations

(20) Microbiota utilization of intestinal amino acids modulates cancer progression and anticancer immunityCell Host & MicrobeJanuary 14, 2026Shanshan Qiao, Ting-Ting Li, Mingeum Jeong, Chuanfa Liu, Sayaka Mizutani, Sung-Min Hwang, Yaxin Li, Mengze Lyu, Kazuhiro Nishiyama, Yu-Ang Tang, et al.Biolegend, 100742), CD8 (PE, Biolegend, 100708), CD45 (BV605, Biolegend, 103140), CD4 (PE-cy7, Biolegend, 100528), CD44 (APC-cy7, CST, 103028), CD62L (BV737, BD Pharmingen™, 612833), PD-1 (BV785, BD Pharmingen™, 744548),

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(1)

8/26/2021Very Bright Fluorophore and Good Antibody Owen JiangReliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.
(1)

4/29/2015Very Useful CD4 Antibody Ferdinando PucciThe aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD4 Antibody from Biolegend ... Read Review

PE/Cyanine7 anti-mouse CD4 from BioLegend

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PE/Cyanine7 anti-mouse CD4 from BioLegend

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