Western Blot: SOX2 Antibody [TA301462] - Detection of SOX2 in mouse brain lysate using TA301462 at 0.5 ug/mL.

Flow Cytometry: SOX2 Antibody [TA301462] - An intracellular stain was performed on A549 with TA301462 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [TA301462] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (TA301462) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.