Immunofluorescence was performed on fixed and permeabilized SH-SY5Y cells for detection of HTT using HTT Recombinant Rabbit Superclonal™ Antibody (Product # 710695, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HTT protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of HTT. Panel e) shows loss of signal by competition with the HTT peptide, demonstrating antibody specificity and panel f) represents control cells with no primary antibody to assess background.