Western blot analysis of anti-Bcl-w BH3 domain Pab (Cat. #TA302168) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody (Cat.#TA302168). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies).
BCLW Antibody (BH3) (RB00972) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BCLW antibody detected the BCLW protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody (Cat.#TA302168), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supplier Page from OriGene Technologies for BCL2L2 Rabbit Polyclonal Antibody
Purification: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.