Fig 1: Immunofluorescent double staining image of GFP-positive bone marrow-derived cell transplantation OSCC mouse model. Most of the F4/80-positive cells merge with GFP-positive cells (arrows), suggesting that most of the TAMs are derived from bone marrow. However, some F4/80 alone-positive cells (arrow heads) are observed, suggesting the presence of yolk sac-derived TAMs in the cancer stromal area. Magnification ×100. Green color; anti-GFP antibody, Abcam, Boston, MA, USA, ab6673, 1:100; red color; anti-F4/80 antibody, BIO RAD, Hercules, CA, USA, C1:A3-1, 1:10.