Fig 2: Abnormal patterning of Taf4a−/− embryos.(a–o) (upper row). In situ hybridization with the indicated probes for anterior visceral endoderm (AVE) and mesoderm markers on E7.5 WT embryos. (b–p) (lower row). In situ hybridization with the indicated probes on E7.5 Taf4−/− embryos. (q–ao). In situ hybridization with neuronal markers in E9.5 WT or Taf4−/− embryos (lateral views (q–ak); ventral view R dorsal views T-AN) and (lateral views RP-AL, higher magnifications of same embryos s–am; ventral view U; dorsal views z–ao). Scale bar, 200 μm in all panels.

Fig 3: Taf4 is required for RA-driven neuronal ES cell differentiation.(a) Phase-contrast microscopy of RA-treated EBs differentiated into neurons 2 days after plating. (b) Tubb3 labelling of RA-treated EBs differentiated into neurons 2 days after plating. (c,d). Immunoblots on extracts from differenting ES cells and EBs with the indicated antibodies. The membranes were stained with Ponceau as loading controls. (e). Immunoblots on extracts from ES cell lines engineered to re-express recombinant Taf4. (f). Phase-contrast microscopy and Tubb3 labelling of RA-treated EBs differentiated into neurons 2 days after plating. All images were taken at × 20 magnification. Scale bars 100 μm.

Fig 4: HNF4A genomic occupancy during liver development.(A) Density cluster profiles of HNF4A ChIP-seq at embryonic day 18.5, P12 and P75. Peaks in the left panel are centred on those occupied at E18.5, in the middle panel on those occupied at P12, and in the right panel at P75. The data for P75 HNF4A occupancy is from Schmidt et al. (2010) [Arrayexpress E-TABM-722]). (B) Western blot of HNF4A expression in 30 µg of nuclear extract from WT embryonic, post-natal, and adult liver. (C) Density cluster profiles of HNF4A ChIP-seq at embryonic day 18.5, P12 and P75 at the sites depleted or enriched in the TAF4 mutant liver (left and right panels, respectively).DOI: http://dx.doi.org/10.7554/eLife.03613.017

Fig 5: Physiopathology of TAF4 inactivation in post-natal hepatocytes.Analysis of serum from WT and Taf4ahep-/- animals for the indicated parameters at P10, N = 6.DOI: http://dx.doi.org/10.7554/eLife.03613.006