Fig 2: S-Nitrosylation of caspase-3 and calpain-1 in reperfused hearts treated with Prolame or SNAP.(A) Immunoblot of immunoprecipitated caspase-3 and (B) S-nitrosylated caspase-3. (C) bars representing the densitometric ratio between SNO procaspase-3/total procaspase-3. Data are mean ± SEM of three independent heart preparations per group. The statistical test used was one-way ANOVA. (D) Immunoblot of immunoprecipitated small subunit of calpain-1 and (E) S-nitrosylated small subunit of calpain-1. (F) bars representing the densitometric ratio between SNO calpain-1/total calpain-1. Data are mean ± SEM of five independent heart preparations per group. The statistical test used was one-way ANOVA.

Fig 3: Aurora B impacts the viability related proteins and genes. (A) The protein levels of p-p38 MAPK, CDK4, PCNA, Fas, caspase-8, caspase-3, Bcl-2 were performed by western blotting. (B) Scanning intensities of western blotting are shown. (C) The mRNA levels of cdk4, PCNA, caspase-3 and bcl-2 were determined using RT-qPCR. Each experiment was repeated three times, and the values are presented as the mean ± standard devidation; *P<0.05, **P<0.01 and ***P<0.001 cf. the 0 µM values. p-p38 MAPK, p-p38 mitogen-activated protein kinase; CDK4, cyclin-dependent kinase 4; PCNA, proliferating cell nuclear antigen; Bcl-2, B-cell lymphoma 2.

Fig 4: OSR upregulates the levels of caspase-3, Bax and cytochrome c expression, and downregulates the levels of Bcl-2 and PARP-1 expression in the transplanted mouse CT26 colorectal cancer tissues. (A and B) The levels of caspase-3, Bax, cytochrome c, Bcl-2 and PARP-1 mRNA expression in the transplanted mouse CT26 colorectal cancer tissues. (C and D) The levels of caspase-3, Bax, cytochrome c, Bcl-2 and PARP-1 protein in transplanted mouse CT26 colorectal cancer tissues.*P<0.05, **P<0.01 vs. the control group. OSR, oxysophoridine; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2 associated X protein; PARP-1, poly (adenosine 5'-diphosphate-ribose) polymerase 1.

Fig 5: Activities of caspases-3, -8, -9 and calpain-1 in reperfused hearts treated with Prolame or SNAP.Activities of caspase-3 (A), -8 (B) and -9 (C) in all experimental groups. All caspase activities were measured as p-nitroaniline (pNA) release with respect to an aniline concentration curve. Data are expressed as mean ± SEM of six independent heart preparations per group. The statistical test used was one-way ANOVA. Caspase-3 activity: *P < 0.05 vs. I-R+SNAP; caspase-8 activity: *P < 0.05 vs. Control and I-R+SNAP; Caspase-9 activity: *P < 0.05 vs. Control and I-R+SNAP. (D) Activity of calpain-1 in all experimental groups. Data are expressed as mean ± SEM of at least nine independent heart preparations per group. The statistical test used was two-way ANOVA. *P < 0.05 vs. all groups.