Gα s (12) Antibody sc-135914 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemGα s (12) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-135914
Antibody TypePrimary Antibodies
Clone12
Quantity200 µg/ml
FormatPurified
Hostmouse
ClonalityMonoclonal
Reactivitymouse, rat, human
Conjugate/TagUnconjugated
ApplicationsWB, IP
NCBI Gene AliasesGSP, PITA3, Oed-Sml, SCG6, PHP1B, Oedsml, NESP, PHP1A, GSA, Nesp55, A930027G11Rik, Nespl, Galphas, Gs-alpha, Nesp, POH, Gnasxl, P2, P3, P1, Gsa, GPSA, C20orf45, C130027O20Rik, GNAS1, 5530400H20Rik, AHO, SgVI, Gnas1
NCBI Full Gene NameGNAS (guanine nucleotide binding protein, alpha stimulating) complex locus, GNAS complex locus
TargetGalpha s
IsotypeIgG1

Questions and Answers

Can this antibody be used to study Gαs protein expression in rat models?

Yes, the Gαs (12) Antibody has been utilized in studies involving rat models, as evidenced by its application in Western blot analysis to assess Gαs protein levels in various experimental contexts, including hypertension research in rats ().

Is there any evidence of this antibody being used in immunohistochemistry?

The Gαs (12) Antibody has been referenced in studies that employed immunohistochemistry techniques, indicating its suitability for visualizing Gαs protein expression in tissue samples ().

What species can this antibody react with, and how has it been validated in research?

The Gαs (12) Antibody is reactive with mouse, human, and rat species. It has been shown to work effectively in various applications, including Western blotting and immunoprecipitation, as documented in multiple scientific articles (, ).

Are there specific experimental conditions under which this antibody has been successfully applied?

This antibody has been successfully applied at a dilution of 1:125 in Western blot experiments, as noted in studies investigating G protein signaling pathways and their implications in conditions such as hypertension and fibrosis (, ).

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) G Proteins-Associated Dose-Dependent Effects of Oxytocin on Oxytocin Neuronal Activity and Astrocytic Plasticity of the Supraoptic NucleusMolecular NeurobiologyJuly 22, 2025Xiao-Yu Liu, Shuo Ling, Yang Liu, Yunhao Jiang, Xiaoran Wang, Shuwei Jia, Chunmei Hou, Yanpeng Li, Delai Qiu, Yu-Feng Wangwith a drop of solution that contained primary antibodies against Gαq/11/14 (G-7, sc-365906), Gαs (sc-135914), and Gαi (sc-515658) from Santa Cruz Biotechnology, Inc., and GFAP (ab4674, 1:500) from Abcam. After

(2) CXCR7 promoted proliferation, migration and invasion in HCC Cells by inactivating Hippo-YAP signalingDiscov OncolApril 18, 2025Genwang Wang, Yu Pang, Nan Li, Yongfeng Hui, Dong JinChina), Gα q/11 (1:500, 41 kDa, Catalog #sc-365906, Santa Cruz, USA), Gα s (1:500, 49 kDa, Catalog #sc-135914, Santa Cruz), phosphorylated (p)-LATS (1:1000, 140 kDa, Catalog #8654S, CST, USA), LATS (1:1000, 140

(3) The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter LesionseNeuroFebruary 13, 2025Chang Cheng, Wenchao Cheng, Yuhan Wang, Xiuying Chen, Lan Zhang, Yi Li, Fa Shen, Dezhi Yuan, Pian Hong, Wen Huangbeads (Santa Cruz Biotechnology) and antibody against Gq or Gs (Santa Cruz Biotechnology, sc-136181 and sc-135914, respectively), overnight at 4°C. The immunocomplexes were washed twice with detergent buffer, and bound

(4) The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter LesionseNeuroFebruary 13, 2025Chang Cheng, Wenchao Cheng, Yuhan Wang, Xiuying Chen, Lan Zhang, Yi Li, Fa Shen, Dezhi Yuan, Pian Hong, Wen Huangbeads (Santa Cruz Biotechnology) and antibody against Gq or Gs (Santa Cruz Biotechnology, sc-136181 and sc-135914, respectively), overnight at 4°C. The immunocomplexes were washed twice with detergent buffer, and bound

(5) Characterisation of a GNAS variant linked to cortisol-producing adrenocortical adenomaEndocr OncolJanuary 1, 2025Jamaluddin A, Wyatt R, Pasaliu A, Ruggles O, Calebiro D, Gorvin CM, Ronchi CL.Tween). Blots were probed with a mouse monoclonal anti-Gαs primary antibody (1:1,000 Gαs antibody (12) sc-135914, Santa Cruz, USA) and an anti-mouse HRP-conjugated secondary antibody (1:3,000; Bio-Rad) and detected

(6) Effects of regulator of G protein signaling 2 (RGS2) overexpression in the paraventricular nucleus on blood pressure in rats with angiotensin II-induced hypertensionFrontiers in PhysiologyJune 21, 2024Shane H. Boomer, Xuefei Liu, Hong Zhengcontrol. The following primary antibodies were used: RGS2 (sc-100761, 1:125); G⍺q (sc-136181, 1:125); G⍺s (sc-135914, 1:125); PKC⍺ (sc-8323, 1:125), PKA⍺ (sc-28315, 1:125), and β-actin (sc-398595, 1:1000) (Santa Cruz Biotechnology

(7) Targeted silencing of GNAS in a human model of osteoprogenitor cells results in the deregulation of the osteogenic differentiation programFront Endocrinol (Lausanne)May 17, 2024Francesca Marta Elli, Deborah Mattinzoli, Masami Ikehata, Francesca Bagnaresi, Maria A. Maffini, Giulia Del Sindaco, Angela Pagnano, Camilla Lucca, Piergiorgio Messa, Maura Arosio, et al.(QuantStudio3, Thermo Fisher Scientific, RRID: SCR_008452). As a starting condition, the Gsα antibody (sc-135914, Santa Cruz Biotechnology, RRID: SCR_008987), raised against Gsα amino acid residues 11–21, after a

(8) Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancerJournal of Experimental & Clinical Cancer ResearchNovember 10, 2023Tianshi Wang, Dehai Wang, Yanan Sun, Ting Zhuang, Xin Li, Huijie Yang, Yifeng Zang, Ziping Liu, Penghe Yang, Chenmiao Zhang, et al.anti-actin (3700, Cell Signaling Technology, 1:1000), anti-Gαq/11 (sc-392, Santa Cruz, 1:1000), anti-Gαs (sc-135914, Santa Cruz, 1:1000), anti-LATS1 (3477, Cell Signaling Technology, 1:1000), and anti-Myc (9E10, Santa

(9) Nrf2/HO-1 partially regulates cytoprotective effects of carbon monoxide against urban particulate matter-induced inflammatory responses in oral keratinocytesCytokineSeptember 1, 2020Cheng CY, Vo TTT, Lin WN, Huang HW, Chuang CC, Chu PM, Lee IT.

(10) Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor DesignCellAugust 6, 2020Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M.

(11) Control of PTH secretion by the TRPC1 ion channelJCI insightApril 23, 2020Onopiuk M, Eby B, Nesin V, Ngo P, Lerner M, Gorvin CM, Stokes VJ, Thakker RV, Brandi ML, Chang W, et al.Glutathione Sepharose 4B (GE Healthcare; GE17-0756-01), respectively. TRPC1 was detected using 1F1 (51), GαS (Santa Cruz; sc-135914), or GST (Santa Cruz; sc-459). Gα11 was detected using FLAG antibody (clone M2, MilliporeSigma; F1804). Immunohistochemistry

(12) Glucocorticoids rapidly activate cAMP production via G(αs) to initiate non-genomic signaling that contributes to one-third of their canonical genomic effectsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyFebruary 1, 2020Nuñez FJ, Johnstone TB, Corpuz ML, Kazarian AG, Mohajer NN, Tliba O, Panettieri RA Jr, Koziol-White C, Roosan MR, Ostrom RS.electroblotting. ß‐actin (Santa Cruz Biotechnology sc‐47778, 1:1000 dilution) and Gαs (Santa Cruz Biotechnology sc‐135914, 1:500 dilution) antibodies were simultaneously incubated overnight at 4°C following block in nonfat

(13) Cannabinoids Exacerbate Alcohol Teratogenesis by a CB1-Hedgehog InteractionScientific reportsNovember 5, 2019Fish EW, Murdaugh LB, Zhang C, Boschen KE, Boa-Amponsem O, Mendoza-Romero HN, Tarpley M, Chdid L, Mukhopadhyay S, Cole GJ, et al.followed by Western blot and ECL detection, and probed with Gαs antibody (~39–45 kDa; 1:500 for 6 hr; #sc-135914 HRP; Santa Cruz Biotechnologies; Dallas, TX), or anti-CB1R polyclonal (~64–66 kDa; 1:750 overnight;

(14) G protein αq exerts expression level-dependent distinct signaling paradigmsCellular signallingJune 1, 2019Kankanamge D, Tennakoon M, Weerasinghe A, Cedeno-Rosario L, Chadee DN, Karunarathne A.non-fat milk, and incubated with the primary antibodies (Santa Cruz Biotechnology) specific for Gαs (sc-135914), Gαq (sc-136181) and α-tubulin (sc-53646), followed by the appropriate HRP-conjugated secondary antibody Cell

(15) G protein-coupled receptors control the sensitivity of cells to the morphogen Sonic HedgehogScience signalingFebruary 6, 2018Pusapati GV, Kong JH, Patel BB, Gouti M, Sagner A, Sircar R, Luchetti G, Ingham PW, Briscoe J, Rohatgi R.

(16) Dibutyryl-cAMP attenuates pulmonary fibrosis by blocking myofibroblast differentiation via PKA/CREB/CBP signaling in rats with silicosisRespiratory researchFebruary 21, 2017Liu Y, Xu H, Geng Y, Xu D, Zhang L, Yang Y, Wei Z, Zhang B, Li S, Gao X, et al.(ab34710, Abcam, Cambridge, UK), Fibronectin (Fn) (ab45688, Eptomics, Burlingame, CA), α-SMA, Gαs (sc-135914, Santa Cruz Biotechnology, Dallas, Texas), Gαi2 (sc-7276, Santa Cruz Biotechnology, Dallas, TX), Gαi3,

(17) Intragenic suppression of a constitutively active allele of Gsα associated with McCune-Albright syndromeJournal of molecular endocrinologyApril 1, 2013Tobar-Rubin R, Sultan D, Janevska D, Turcic K, Carroll J, Ooms L, Pals-Rylaarsdam R.

(18) Role of TLR4/NADPH oxidase/ROS-activated p38 MAPK in VCAM-1 expression induced by lipopolysaccharide in human renal mesangial cellsCell communication and signaling : CCSNovember 15, 2012Lee IT, Shih RH, Lin CC, Chen JT, Yang CM.

(19) Adenosine triphosphate regulates NADPH oxidase activity leading to hydrogen peroxide production and COX-2/PGE2 expression in A549 cellsAmerican journal of physiology. Lung cellular and molecular physiologySeptember 1, 2012Lin CC, Lee IT, Wu WL, Lin WN, Yang CM.

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1/25/2016G alpha s Antibody for Western Blotting Ganesh PusapatiThe Ga s subunit of the heterotrimeric G proteins stimulates adenylate cyclases to generate cAMP, an important second messenger molecule that is necessary for PKA activation. The goal of this ... Read Review

Gα s (12) Antibody

Gα s (12) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Gα s (12) Antibody from Santa Cruz Biotechnology, Inc.

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