Fig 1: PGC-1α promotes the differentiation and survival of newborn neurons in the DG of AD hippocampus. (A) Three weeks after the stereotaxic injection of the AAV-Control or AAV-PGC-1α into the APP/PS1 mice, EdU was administered intraperitoneally. Two weeks later, (B) hippocampal sections were immunolabeled with EdU (Green), DCX (Red), and DAPI (Blue). GCL, granule cell layer. (b1) Short-term survival of immature neurons is quantified as EdU+; DCX+/DAPI+ cells (%). (b2) DCX protein density (cells/mm2) was measured in the DG. (C) Three weeks after AAV injection, EdU was administered. Six weeks later, (D) hippocampal sections were immunolabeled with EdU (Green), NeuN (Red), and DAPI (Blue). (d1) Long-term survival of mature neurons is quantified as EdU+; NeuN+/DAPI+ cells (%). (d2) NeuN protein density (cells/mm2) was measured in the DG. Values are means ± S.E.M., n = 6/group. Fisher’s LSD test: **p < 0.01, ***p < 0.001 for comparisons between AAV-Control and AAV-PGC-1α groups.