AMV Reverse Transcriptase (HC) from Promega

Product Specs
ItemAMV Reverse Transcriptase (HC)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM9004
Quantity600u
Concentration20–25 U/µl

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml BSA, 1mM radiolabeled dTTP and 0.25mM poly(A)400 and 0.25mM oligo (dT)50.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) RNA 2'-O-methylation promotes persistent R-loop formation and AID-mediated IgH class switch recombinationBMC BiologyJuly 8, 2024Muzaffer Ahmad Kassab, Yibin Chen, Xin Wang, Bo He, Eric J. Brown, Xiaochun Yuthen carried reverse transcription at elevated temperatures (68 °C) using AMV RT (Promega cat. no. M9004). The presence of 2′-O-methylated bases leads to a strong bump in reverse transcriptase, and a dominant

(2) RNA binding proteins PTBP1 and HNRNPL regulate CFTR mRNA decayHeliyonNovember 13, 2023Amna Siddiqui, Arpit Saxena, Joshua Echols, Viktoria Havasi, Lianwu Fu, Kim M. KeelingRNasin (Promega N2511); 10 μL of 5X AMV RT buffer and 40 U/μL AMV reverse transcriptase (Promega PR-M9004). Reverse transcription (RT) reactions were incubated at 42 °C for 1.5 h and then heat inactivated at

(3) Triamterene Functions as an Effective Nonsense Suppression Agent for MPS I-H (Hurler Syndrome)International Journal of Molecular SciencesFebruary 24, 2023Amna Siddiqui, Halil Dundar, Jyoti Sharma, Aneta Kaczmarczyk, Josh Echols, Yanying Dai, Chuanxi Richard Sun, Ming Du, Zhong Liu, Rui Zhao, et al.40U RNasin (Promega N2511); 10 mL of 5× AMV RT buffer and 40U AMV reverse transcriptase (Promega PM-M9004). RT reactions were incubated at 42 °C for 1.5 h, and then heat-inactivated at 65 °C for 15 min. The

(4) Immunonutritional Protease Inhibitors from T. durum and A. sativa Display Metabolic Similarities When Assayed on Human Macrophage-like CellsInternational Journal of Molecular SciencesAugust 2, 2021Bartosz Fotschki, Aurora Garcia Tejedor, Juan Antonio Nieto Fuentes, Jose Moisés Laparra Llopisinstructions. Total RNA (1 μg) was converted to double-stranded cDNA using AMV Reverse Transcriptase (Cat No. M9004 Promega, Madison, USA). PCR was performed with primers designed (www.ncbi.nlm.nih.gov, accessed on 25

(5) The transcriptional landscape of the cultured murine middle ear epithelium in vitroBiology OpenApril 15, 2021Apoorva Mulay, Md Miraj K. Chowdhury, Cameron T. James, Lynne Bingle, Colin D. BingleNo-M6101) and 200 ng of RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, cat. No-M9004). RT-PCR was performed with 1 µl of template cDNA and Maxima Hot Start Green PCR Master Mix (Thermo Fisher Scientific

(6) High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseqMolecular CellApril 15, 2021Andrew Behrens, Geraldine Rodschinka, Danny D. NedialkovaIn,Ambion,Cat# AM2694 TGIRT,InGex,Cat# TGIRT50 Superscript III,Invitrogen,Cat# 18080044 AMV RT,Promega,Cat# M9004 CircLigase ssDNA ligase,Lucigen,Cat# CL4115K KAPA HiFi DNA Polymerase,Roche,Cat# KK2102 DNA Clean&Concentrator-5

(7) Transcriptional landscape of the embryonic chicken Müllerian ductBMC GenomicsDecember 1, 2020Zahida Yesmin Roly, Rasoul Godini, Martin A. Estermann, Andrew T. Major, Roger Pocock, Craig A. Smithreversed transcribed into cDNA using random hexamers using AMV reverse transcriptase (Promega, Cat. # M9004). For RT-PCR, gene-specific primers were used as shown in Supplementary Table 1. RT-PCR was performed

(8) Prevalence and risk factors of Occult Hepatitis C infections in blood donors from Mexico CityPLOS ONEOctober 19, 2018María de la Luz Martínez-Rodríguez, Luis A. Uribe-Noguez, Carla I. Arroyo-Anduiza, José Antonio Mata-Marin, Gamaliel Benitez-Arvizu, María L. Portillo-López, Alicia Ocaña-Mondragónobtained by RT-PCR using the AMV Reverse Transcriptase kit and random hexamer primers (Catalog number: M9004, Promega, Wisconsin, USA) following the manufacturer’s instructions. Nested PCR The 5’-UTR region of

(9) Raspberry Polyphenolic Extract Regulates Obesogenic Signals in HepatocytesMoleculesAugust 21, 2018Bartosz Fotschki, José Moisés Laparra, Michał SójkaTotal RNA (0.45 µg) was converted to double-stranded cDNA using AMV Reverse Transcriptase (Cat No. M9004 Promega, Madison, WI, USA). PCR was performed with primers designed (www.ncbi.nlm.nih.gov) for the following

(10) An in vitro model of murine middle ear epitheliumDisease models & mechanismsNovember 1, 2016Mulay A, Akram KM, Williams D, Armes H, Russell C, Hood D, Armstrong S, Stewart JP, Brown SD, Bingle L, et al.(Promega, M6101) and 200 ng of RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, M9004). RT-PCR was performed with 1 µl of template cDNA and Maxima Hot Start Green PCR Master Mix (Thermo Fisher Scientific

(11) Methodology for the High-Throughput Identification and Characterization of tRNA Variants That Are Substrates for a tRNA Decay PathwayMethods in EnzymologyAugust 9, 2015Matthew J. Payea, Michael P. Guy, Eric M. Phizicky1mM dTTP, 1mM ddCTP, and 2 units AMV Reverse Transcriptase in the provided buffer (Promega, Cat# PR-M9004)) to the annealed reaction on ice and then incubate for 1 hour at 50°C, prior to storage of reaction

(12) Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobiaBMC Plant BiologyDecember 1, 2014Araceli Nora García, Nicolás Daniel Ayub, Ana Romina Fox, María Cristina Gómez, María José Diéguez, Elba María Pagano, Carolina Andrea Berini, Jorge Prometeo Muschietti, Gabriela Sotowas obtained using random hexamers (Cat. # B070-40, Promega) and AMV Reverse Transcriptase (Cat. # M9004, Promega). For PCR amplification, 1 μl of RT reaction was used. The PCR reactions were carried out in

(13) The Effects of Cigarette Smoke Extract on Ovulation, Oocyte Morphology and Ovarian Gene Expression in MicePLoS ONEApril 28, 2014Zixin Mai, Ming Lei, Bolan Yu, Hongzi Du, Jianqiao Liu0.5 ml of Recombinant Rnasin Ribonuclease Inhibitor, 15 u of AMV Reverse Transcriptase (HC; Promega M9004; Madison USA), 0.5 mg of Oligo(dT)15 Primer, and nuclease-free water to a final volume of 20 ml. The

(14) Correction: Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient ConditionsPLoS ONEOctober 30, 2013Lorena Setten, Gabriela Soto, Matteo Mozzicafreddo, Ana Romina Fox, Christian Lisi, Massimiliano Cuccioloni, Mauro Angeletti, Elba Pagano, Antonio DÃaz-Paleo, NicolÃ¡s Daniel Ayubtreated with DNaseI. cDNA was obtained using random hexamers (Promega) and AMV Reverse Transcriptase M9004 (Promega) following the manufacturer’s instructions. For PCR amplification, 1 ml of RT reaction was

(15) Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient ConditionsPLoS ONEMay 13, 2013Lorena Setten, Gabriela Soto, Matteo Mozzicafreddo, Ana Romina Fox, Christian Lisi, Massimiliano Cuccioloni, Mauro Angeletti, Elba Pagano, Antonio Díaz-Paleo, Nicolás Daniel Ayubtreated with DNaseI. cDNA was obtained using random hexamers (Promega) and AMV Reverse Transcriptase M9004 (Promega) following the manufacturer’s instructions. For PCR amplification, 1 ml of RT reaction was

(16) Enhanced proliferation of human skeletal muscle precursor cells derived from elderly donors cultured in estimated physiological (5%) oxygenCytotechnologyDecember 1, 2009Sheree D. Martin, Fiona M. Collier, Mark A. Kirkland, Ken Walder, Nicole Stupkainstructions. Complimentary DNA was synthesized by reverse transcription using AMV Reverse Transcriptase (#M9004, Promega) and random hexamer primers (#1181, Promega). Real-time PCR To assess cyclophilin, myogenin,

(17) Prostaglandin E 2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: An in vivo and in vitro studyProstaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)January 7, 2006G.O. Ramirez-Yañez, S. Hamlet, A. Jonarta, G.J. Seymour, A.L. Symons

(18) Cancer cell adaptation to chemotherapyBMC CancerDecember 1, 2005Federica Di Nicolantonio et al.recombinant RNasin® ribonuclease inhibitor and 15 units of reverse transcriptase AMV (Promega, Cat No. M9004) to each 20 μl reaction. The resulting c-DNA was amplified by qPCR on a Biorad iCycler instrument (BioRad

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