MagneHis Ni-Particles from Promega

Product Specs
ItemMagneHis Ni-Particles
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8565
Quantity10ml
Product TypeResins, Magnetic Beads
ModeAffinity Purification, Metal Chelate
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A Fusarium sacchari Glycoside Hydrolase 12 Protein FsEG1 Is a Major Virulence Factor During Sugarcane Infection and Confers Resistance to Pokkah Boeng Disease via the HIGS StrategyPlant Biotechnology JournalFebruary 4, 2026Deng Wu, Tianshu Hong, Lulu Wang, Qianqian Ren, Shichao Wang, Yixue Bao, Muqing Zhang, Wei Yao, Qin Hurecombinant protein was purified using MagneHis Protein Purification System (Promega, Madison, USA, V8565) according to the manufacturer's instructions, the immunoblot of the purified protein was performed

(2) Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel NetworkACS NanoJanuary 20, 2026Matthew E. Allen, James W. Hindley, Maya I. Müller, Kexin Cai, Marina K. Kuimova, Robert V. Law, Simon D. Connell, Seraphine V. Wegner, Oscar Ces, Yuval Elani3 mM in chloroform. Rhodamine B-labeled alginate was purchased from HAworks (New Jersey, USA). The MagneHis Ni-Particles were obtained from Promega (Southampton, UK). All other reagents including sodium alginate, chloroform,

(3) [64Cu]Cu-NOTA-EV-F(ab′)2 Enables Same-Day Immuno-PET Imaging of Nectin-4 in Triple-Negative Breast and Urothelial Bladder CancersJournal of Nuclear MedicineSeptember 2, 2025Wenpeng Huang, Liming Li, Fangfang Chao, Qi Yang, Jason C. Mixdorf, Jonathan W. Engle, Jessica C. Hsu, Lei Kang, Weibo Cairegion. The Fc fragments were then separated from the F(ab′)2 fragments using Magne Protein A beads and MagneHis Ni particles (Promega Corporation). Subsequently, the purified F(ab′)2 fragments were conjugated with the NOTA chelator

(4) A novel MAP7D1 mutation causes mitotic defects and RPS14 accumulation in Shwachman−Diamond syndrome patient cellsDisease Models & MechanismsAugust 1, 2025Seren Kucukvardar, Arzu Karabaylysate obtained from HEK293T cells overexpressing wild-type or mutant MAP7D1 were incubated with 35 µl MagneHis™ Ni particles and binding/wash buffer (100 mM HEPES, 10 mM imidazole, 500 mM NaCl) on a rotator at 4°C for 4 h. After

(5) ImmunoPET imaging of Trop2 expression in triple-negative breast cancer using [64Cu]Cu-NOTA-Trodelvy-F(ab’)2European Journal of Nuclear Medicine and Molecular ImagingJuly 1, 2025Wenpeng Huang, Yuwei Zhang, Xiaoyan Xiao, Qi Yang, Jason C. Mixdorf, Xinyao Sun, Jonathan W. Engle, Yu Fan, Liming Li, Lei Kang, et al.supernatant, containing purified F(ab’)2 fragments, was collected. To ensure further purification, MagneHis™ Ni Particles (Promega) was added to the digested sample, and the supernatant containing the desired F(ab’)2 fragments

(6) The Function of TER94 of Spodoptera frugiperda 9 Cells When Infected With Invertebrate Iridescent Virus‐6Journal of Basic MicrobiologyMay 13, 2025Kubra Zengin, Cihan Inan, Remziye Nalcacioglu, Zihni Demirbagincubated at 4°C for 3 h with gentle shaking. His‐tagged protein (TER94) was purified using nickel beads (MagneHis™ Ni‐Particles, Promega). Samples were separated on 12% SDS‐PAGE and analyzed by western blot analysis using an anti‐GST

(7) High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet NetworksAdvanced MaterialsApril 30, 2025Jorin Riexinger, Thomas Caganek, Xingzao Wang, Yutong Yin, Khoa Chung, Linna Zhou, Hagan Bayley, Ravinash Krishna Kumaradding a preheated (60 °C) solution (composition not stated by manufacturer) containing Ni‐particles (MagneHis Ni‐Particles, Promega) to preheated (60 °C) M9 medium containing 1.5% w/v of ULGA at a volume ratio of 2:3. Droplets

(8) Microfluidic Production of Spatially Structured Biomimetic Microgels as Compartmentalized Artificial CellsSmall ScienceApril 1, 2025Matthew E. Allen, James W. Hindley, Robert V. Law, Oscar Ces, Yuval Elanisalt) (Biotin‐PE) were purchased from Avanti Polar Lipids (Alabaster, AL) as chloroform stocks. The MagneHis Ni‐Particles were obtained from Promega (Southampton, UK). PDMS Sylgard 184 elastomer kits were purchased from Dow

(9) Symptomatology and IgG Levels before and after SARS-CoV-2 Omicron Breakthrough Infections in Vaccinated IndividualsVaccines (Basel)October 8, 2024Nigella M. Paula, Emerson Joucoski, Valter A. Baura, Emanuel M. Souza, Fabio O. Pedrosa, Alan G. Gonçalves, Luciano F. Huergoanalysis, 1 mg of His-tagged antigens was incubated with 1 mL of nickel magnetic particles (Promega-V8565) in 50 mL of TBST for 5 min at room temperature with gentle mixing. The beads were washed with 25 mL

(10) Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applicationsMolecular CellFebruary 1, 2024Chunyi Hu, Mason T. Myers, Xufei Zhou, Zhonggang Hou, Macy L. Lozen, Ki Hyun Nam, Yan Zhang, Ailong KeColumn,Cytiva,17116601 HiPrep Sephacryl S-300 HR Column,Cytiva,17116701 MagneHis Ni Particles,Promega,V8560 Mix & Go E. coli Transformation Kit,Zymo Research,T3001 Quantifoil holey carbon grid,Quantifoil,191108 Deposited

(11) Beyond structural bioinformatics for genomics with dynamics characterization of an expanded KRAS mutational landscapeComputational and Structural Biotechnology JournalOctober 5, 2023Brian D. Ratnasinghe, Neshatul Haque, Jessica B. Wagenknecht, Davin R. Jensen, Guadalupe K. Valdivia Esparza, Elise N. Leverence, Thiago Milech De Assuncao, Angela J. Mathison, Gwen Lomberk, Brian C. Smith, et al.workgroups of 12, using the Maxwell 16 (Promega), utilizing magnetic nickel affinity beads (Promega #V8565) in a semi-automated nickel affinity purification strategy. The Maxwell 16 has a cassette with seven

(12) YgfB increases β-lactam resistance in Pseudomonas aeruginosa by counteracting AlpA-mediated ampDh3 expressionCommunications BiologyMarch 10, 2023Ole Eggers, Fabian A. Renschler, Lydia Anita Michalek, Noelle Wackler, Elias Walter, Fabian Smollich, Kristina Klein, Michael S. Sonnabend, Valentin Egle, Angel Angelov, et al.proteins 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 µM was incubated with 100 µl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5, 25 mM imidazole, 300 mM NaCl, 0.5% IGEPAL,

(13) Population-Based Analysis of the Immunoglobulin G Response to Different COVID-19 Vaccines in BrazilVaccines (Basel)December 22, 2022Nigella M. Paula, Marcelo S. Conzentino, Ana C. A. Gonçalves, Renata da Silva, Karin V. Weissheimer, Carlos H. S. Kluge, Paulo H. S. A. Marins, Haxley S. C. Camargo, Lucas R. P. Farias, Thamyres P. Sant’Ana, et al.described previously [15,16,17,18,19] were incubated with 1 mL of nickel magnetic particles (Promega—V8565) in 50 mL of TBST. After 5 min at room temperature with gentle mix, the beads were washed with 25 mL

(14) Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum sensing transcriptional regulationStructureDecember 1, 2022J. Ryan Feathers, Erica K. Richael, Kayla A. Simanek, J. Christopher Fromme, Jon E. Paczkowskifrom PqsE- and RhlR-containing cells were combined, with 20 μL saved for input assessment. Promega MagneHis™ Ni-Particles, (20 μL and 40 μL per sample for PqsE variants and RhlR variants, respectively) were washed with lysis

(15) Development of an RNA-protein crosslinker to capture protein interactions with diverse RNA structures in cellsRNA (New York, N.Y.)March 1, 2022Han Y, Guo X, Zhang T, Wang J, Ye K.adjusted to contain 6 M guanidine HCl, 300 mM NaCl and 10 mM imidazole and incubated with 30 µL of MagneHis Ni-Particles (Promega) at 4°C for 12 h. The beads were washed with wash buffer (20 mM HEPES-Na [pH 7.5], 6 M Guanidine

(16) XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coliSTAR ProtocolsDecember 17, 2021Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. Nickels100K Centrifugal Filters,Thermo Fisher Scientific,Cat#UFC810024 MagneHis Ni Particles,Promega,Cat#V8560 Promega MagneSphere Magnetic Separation Stand,Promega,Cat#Z5343 "Spin-X centrifuge tube filter, 0.45

(17) Functional characterization of NADPH-cytochrome P450 reductase and cinnamic acid 4-hydroxylase encoding genes from Scoparia dulcis L.Botanical StudiesDecember 1, 2020Yoshimi Yamamura, Ayaka MabuchiReagent (Novagen-Merck Millipore) and His-tag recombinant proteins were purified from the supernatant using MagneHis Ni-Particles (Promega) with elution buffer containing 1 M imidazole. Recombinant enzyme assays The activities of

(18) XACT-Seq Comprehensively Defines the Promoter-Position and Promoter-Sequence Determinants for Initial-Transcription PausingMolecular CellSeptember 1, 2020Jared T. Winkelman, Chirangini Pukhrambam, Irina O. Vvedenskaya, Yuanchao Zhang, Deanne M. Taylor, Premal Shah, Richard H. Ebright, Bryce E. Nickelsphenol:chloroform (CHCl3) pH 4.5,Thermo Fisher,AM9720 Ni-NTA agarose,Qiagen,30230 MagneHis Ni-Particles,Promega,V8560 DNase I,Zymo Research,E1009A T4 RNA Ligase 1 (ssRNA Ligase),NEB,M0204L 5’ App DNA/RNA ligase,NEB,M0319S T4

(19) Thioglycosides Are Efficient Metabolic Decoys of Glycosylation that Reduce Selectin Dependent Leukocyte AdhesionCell chemical biologyDecember 20, 2018Wang SS, Gao X, Solar VD, Yu X, Antonopoulos A, Friedman AE, Matich EK, Atilla-Gokcumen GE, Nasirikenari M, Lau JT, et al.volume was increased 10-fold using 500 mM NaCl in phosphate-buffered saline (PBS) and mixed with 10 μL MagneHis™ Ni-Particles (Promega, Madison, WI) for 45 min at r.t.. Followed 5-wash cycles in washing buffer (100 mM NaCl, 10

(20) A Synthetic Cross-Species CD200R1 Agonist Suppresses Inflammatory Immune Responses In VivoMolecular Therapy - Nucleic AcidsSeptember 7, 2018Aaron Prodeus, Amanda Sparkes, Nicholas W. Fischer, Marzena Cydzik, Eric Huang, Ismat Khatri, Ashley Young, Lindsay Woo, Chung Wai Chow, Reginald Gorczynski, et al.oligonucleotides. The library (4 nmol; theoretical diversity: 425 or ∼1.1 × 1015 sequences) was pre-adsorbed onto MagneHis Ni-Particles (Promega, Fitchburg, WI, USA) at 37°C for 1 hr to remove sequences that bound to the solid support itself.

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