Time-Resolved Forster Resonance Energy Transfer (TR-FRET) is a cutting-edge immunoassay technique widely used for the detection and quantification of biomolecules. This homogeneous method is fast, wash-free, reduces background fluorescence, and is cost-effective. High-brightness nanoparticles and fluorophores to further improve the limits of detection and sensitivity compared to traditional TR-FRET methods, and additional developments facilitate the simultaneous detection of multiple targets.
In this application note from Molecular Devices, learn more about how a multi-mode microplate reader can perform a robust, no-wash immunoassay that detects two targets in the same well.