The past few decades have witnessed major advances in cancer research, alongside innovations leading to new and increasingly personalized treatments. Nonetheless, given the global impact of the disease, a substantial share of cancer research focuses on understanding the molecular, biochemical, and immunological mechanisms that drive malignant transformation and tumor progression. Tumor cells exhibit complex, heterogeneous, and dysregulated gene expression programs that reveal much about their underlying biology. Tumor expression profiling is thus critical to correlating biomarkers with their biological roles and revealing cancer-relevant signaling pathways and axes.

In this application note from Thermo Fisher Scientific, discover how gene expression pathway analysis can define important cancer pathways and axes involved in tumorigenesis and cancer progression, and the role real-time PCR plays in enabling these discoveries.