Colorectal cancer is a high disease burden cancer and is the second leading cause of cancer deaths worldwide. As such, novel therapeutics are in demand, especially for recurrent disease. Here, a deeper understanding of the tumor microenvironment of colon cancer tissues is imperative, and spatial biology approaches can provide scientists with a way to gather those insights.
In this application note from Leica, explore a 3D, high-multiplex confocal imaging workflow using a 30-biomarker panel to study the dynamics of immune and cancer cells within colon cancer tissues.