Alzheimer’s disease (AD) is the most common neurodegenerative disease in humans. While characterized by the progressive decline of cognitive function, the specific molecular and cellular mechanisms driving these phenotypic changes are varied and difficult to parse. Understanding the spatial microenvironment of AD brain tissue remains a critical challenge. Yet this type of spatial profiling is crucial for uncovering cellular relationships that could provide insights into disease mechanisms and potential therapeutic strategies.
In this application note from Leica, discover a confocal microscopy workflow that combines cell-type specific biomarker detection, multiplexed tissue imaging, and AI-guided analysis that sheds more light on the spatial heterogeneity in the AD brain.