CRYO-DMSO-F is a chemically defined cryopreservation medium designed for the preparation, and preservation of cells under ultra-low temperature environments. Unlike conventional cryopreservation media that rely heavily on dimethyl sulfoxide (DMSO) as a cryoprotectant, this formulation is DMSO-free, serum-free, protein-free, and animal origin-free. The medium has been developed to offer a safe and protective environment for cells during the complete cryopreservation workflow, with effective performance demonstrated across a wide temperature range from -80°C to -196°C.
One of the key challenges in cryopreservation is mitigating the damage caused to cells during the freezing and thawing process. Freezing can induce apoptosis and necrosis, reducing post-thaw cell viability and limiting functional recovery of the cultures. CRYO-DMSO-F addresses this problem by providing a specialized formulation that reduces freezing-induced cell death, thereby helping to maintain both cell integrity and function.
DMSO has played a central role in cell cryopreservation for decades, but its toxicity is well documented. Some cell types are less affected, while others—particularly sensitive cell populations—show significantly compromised viability upon exposure. Toxicity is most pronounced during the thawing phase, when prolonged exposure to DMSO can impact both post-thaw cell viability as well as the overall functionality of the cells themselves. CRYO-DMSO-F overcomes this limitation by eliminating the need for DMSO in its formulation. The removal of DMSO ensures better viability and phenotypic maintenance for sensitive cell types. Stem cells, CAR-T cells, NK cells, and macrophages are all examples of populations that benefit from avoiding exposure to DMSO, where post-thaw quality is essential for experimental reliability and therapeutic application.
The fully defined composition of CRYO-DMSO-F supports broad use across research and therapy-related cell storage needs. It is particularly well suited for immune cell therapy, primary cell preservation, stem cell banking, and general cell storage. Its chemically defined nature eliminates variability introduced by serum or protein components, enabling more standardized cryopreservation conditions. By avoiding animal-origin materials, the medium also reduces risks associated with contamination and immune reactivity, an important consideration in regulated environments.
Ease of use has been built into the design of CRYO-DMSO-F. The formulation is complete and ready to use, removing the need for additional media preparation steps. This streamlines the freezing workflow, reduces handling time, and lowers the risk of user error during preparation. The solution is sterile filtered, helping to minimize contamination and ensuring reliability even for highly sensitive stem cell cultures. When not in use, it is stored at 4°C for convenience, eliminating the waiting periods or planned thawing steps required with certain formulations.
Overall, CRYO-DMSO-F represents an approach to cell cryopreservation that balances safety, effectiveness, and practicality. By moving away from DMSO while maintaining strong protective performance under freezing and thawing conditions, the medium supports high post-thaw viability and function across a wide range of cell types. Its defined, serum-free formulation helps ensure reliability and reproducibility in workflows where cell quality cannot be compromised, making it suitable for both experimental research and applied therapeutic cell storage.