In most conventional labs, cells are cultured under normoxic conditions, meaning the oxygen level is at 21%, matching atmospheric oxygen. However, this does not reflect the natural environment of many human cell types. Consequently, cells cultured under normoxia can experience stress and altered metabolism. In contrast, culturing cells under physioxia is known to enhance viability, function, and differentiation, ensuring more reliable results.
This eBook looks at how researchers’ understanding of hypoxia has developed over the years, before delving into the critical importance of physioxia for stem cell culture, neuronal cell culture, and the generation of adoptive cell therapies.