Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, is one of the most widely used techniques in molecular biology. Due to its high sensitivity, qPCR is often used for gene expression analysis, genetic testing, mutation detection, and pathogen identification. This eBook provides a detailed view into the core concepts of qPCR, while presenting innovative approaches for streamlining experiments and improving experimental accuracy.

Topics covered in this eBook include:
- Tips on streamlining experiments
- SYBR™ Green vs TaqMan™
- Automated master mix preparation
- qPCR plate set-up
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