Blocking Peptides

Blocking peptide reagents are typically produced through synthetic methods, where specific sequences of amino acids are assembled to create peptides useful in protein studies. In immunostaining applications like immunohistochemistry, blocking peptides can be used to block specific antibody-antigen interactions to help reduce non-specific binding and improve the specificity of antibody staining. In Western blots, they can be used to confirm the specificity of antibody binding. They can also be used in functional assays to inhibit protein activity when studying protein functions, interactions, or related biochemical pathways. Synthetic blocking peptides are usually synthesized using solid-phase peptide synthesis, purified by HPLC, and subsequently characterized to confirm identity and purity. Visit the peptide supplier page for more information, including peptide sequence, species reactivity, and validated applications.