Antigen Retrieval Buffers

Antigen Retrieval buffers and solutions are primarily used for unmasking antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Antigen retrieval is a crucial step in immunohistochemistry (IHC) as it restores antigen epitopes obscured by crosslinking from the fixation process. These reagents are important for enabling antibodies to effectively bind their target antigens. Antigen Retrieval buffers are available in different formulations, commonly based on pH levels (acidic, neutral, or alkaline), tailored to specific antigen requirements. When choosing a buffer, consider the type of antigen, compatibility with your antibodies, and the tissue type. Additionally, evaluate whether the buffer is pre-made or requires preparation, balancing convenience with cost. Pre-tested buffers with clear protocols can help with planning downstream experiments and improve reproducibility. Visit the supplier page for product datasheets and purchasing information.