Cell Cycle Assay kits include essential reagents to measure various stages of cell growth and division, including mitosis (G1, S, G2, M) and apoptosis. Cell cycle assays generally work by staining cellular DNA with a fluorescent dye, which binds DNA in proportion to its content. After staining, cells are analyzed via flow cytometry, where the fluorescence intensity helps determine the percentage of cells in various phases (such as G0/G1, S, G2/M, and sub-G1). The assay allows researchers to monitor cell cycle progression and detect proliferating or apoptotic cells. When selecting a cell cycle assay kit, researchers should consider factors like cell type, compatibility with culture media, and whether the kit is suited for live or fixed cells. Visit the supplier page for more details, including kit components and assay protocols.
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- Detection cell cycle and cell proliferation using flow cytometry
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