CCK-8 Assay Kits

CCK-8 (Cell Counting Kit-8) assay kits contain reagent sets to perform a dye-based method for measuring cell viability and proliferation. The assay is based on the reduction of a water-soluble tetrazolium salt WST-8 (2-[2-methoxy-4-nitrophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H-tetrazolium) by cellular dehydrogenases in viable cells. The reaction produces an orange-colored formazan dye, which can be measured using a microplate reader at a wavelength of 450 nm. The change in color intensity is directly proportional to the number of viable cells. The procedure is sensitive, straightforward, and can be completed in a few hours, making it suitable for high-throughput screening. The CCK-8 assay is widely used in studies assessing cell viability and proliferation, cytotoxicity testing, and monitoring cell growth and health in response to various treatments and compounds. When choosing an assay kit, consider important specifications such as the recommended sample formats, and the components included within the kit. Visit the supplier page for more details.