COMET ™ from Bio-Techne Spatial

Product Specs
ItemCOMET ™
CompanyBio-Techne Spatial
Price Get Pricing Get Quote for this Product
Catalog NumberCOMET
MethodSequential immunofluorescence (SeqIF™); automated runs of 40-plex
ScaleWhole tissue
ApplicationsMultiplex, Sequential immunofluorescence (seqIF™)
TypeAutomated Imager
QuantityEA

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Bio-Techne Spatial

Route de Lully 5c

1131 Tolochenaz

Switzerland

Company Profile

Website: bio-techne.com/spatial

(1) Targeting the epithelium in pulmonary fibrosisEur Respir RevMarch 25, 2026Muriel Lizé, Christoph H. Mayr, Marina Bleck, Lutz Wollin, Christian T. Wohnhaas, Irina Shalashova, Heiko Olbrich, Ralf Heilker, Anna Engler, Marie-Therese Bammert, et al.terminal bronchial secretory cells (TB-SCs) and MMP7+ airway cells. c) Schematic overview of the Lunaphore COMET sequential hyperplex immunofluorescence workflow. d) Healthy and e) idiopathic pulmonary fibrosis (IPF)

(2) Toward Computationally Complete Spatial OmicsbioRxivMarch 5, 2026Wei Li, Liran Mao, Yunhe Liu, Fuduan Peng, Nadja Sachs, Wenrui Wu, Stephanie Pei Tung Yiu, Hanying Yan, Amelia Schroeder, Xiaokang Yu, et al.v8.0.0) based on the high-resolution H&E images. Automated seqIF-based spatial proteomics and imaging on COMET. Sequential immunofluorescence (seqIF) was conducted using the COMET platform (Lunaphore Technologies).

(3) Deep Learning Enabled 3D Multi-Omic Analysis Reveals Molecular Signatures of Heterogeneous Response to Chemotherapy in Pancreatic CancerbioRxivMarch 5, 2026André Forjaz, Hengameh Mojdeganlou, Alens Valentin, Meredith Wetzel, Dmitrijs Lvovs, Atul Deshpande, Sarah M. Shin, Sujan Piya, Kimal I Rajapakshe, Paola A Guerrero, et al.generated using ggplot and igraph. seqIF data generation and analysis Prior to seqIF staining on the COMET (Lunaphore) platform, formalin-fixed paraffin-embedded (FFPE) slides were preprocessed for autofluorescence

(4) STING activation induces cytotoxic and immune responses in meningiomas via inflammatory cell death pathwaysNature CommunicationsFebruary 12, 2026Mark W. Youngblood, Shashwat Tripathi, Hinda Najem, Harrshavasan Congivaram, Mateo Gomez, Thomas K. Sears, Lisa Hurley, Leah K. Billingham, Caylee Silvers, Wenxia Wang, et al.runs of the multiplex panel were executed using the seqIF™ methodology integrated into the Lunaphore COMET™ platform (characterization 2 and 3 protocols, and seqIF™ protocols, respectively)97. The staining is

(5) Uncovering the signatures of aging and senescence in the human dorsolateral prefrontal cortexCell GenomicsFebruary 11, 2026Nicholas X. Sloan, Jason Mares, Aidan C. Daly, Shaunice Grier, Imdadul Haq, Christopher A. Jackson, Natalie Barretto, Obadele Casel, Kristy Kang, Shruti Khiste, et al.nuclei. Select senescence-related DEGs display correlated changes in their protein expression Using Lunaphore’s COMET platform,66 we performed multiple cycles of immunostaining and elution on the same tissue section from

(6) Integrated immune profiling of chordomas reveals spatially organized niches and functional heterogeneityNeuro-OncologyFebruary 1, 2026Victor A Arrieta, J Kada Benotmane, Rebecca Du, Karl J Habashy, Junfei Zhao, Hinda Najem, Si Wang, David Hou, Joshua L Katz, Gustavo I Vázquez-Cervantes, et al.(seqIF) Six tumor specimens were used for sequential multiplex immunofluorescence using the Lunaphore’s COMET platform as detailed in Supplementary material. Methods for k-Nearest Neighbor (kNN) Analysis of Immune

(7) Integrating spatial omics with routine haematoxylin and eosin in formalin-fixed paraffin-embedded: a step-by-step clinical workflowF1000ResearchJanuary 19, 2026Nasar AlwahaibiMultiplexed Ion Beam Imaging (MIBI), 24, 25 or cyclic immunofluorescence systems such as Lunaphore COMET, MACSIMA, PhenoCycler Fusion (formerly CODEX) and CyCIF; 26 – 28 these often align naturally with

(8) Spatial omics at the forefront: emerging technologies, analytical innovations, and clinical applicationsCancer CellJanuary 12, 2026Yunhe Liu, Yibo Dai, Linghua Wangof 40-plus markers using metal-conjugated antibodies with laser ablation and mass spectrometry. The COMET platform (Lunaphore) enables rapid, flexible up to ~40-plex mIF panels using standard label-free antibodies. These

(9) IBA-1+CD68+ Germinal Center Macrophages Harbor Proviral and Inducible Clade C HIV Reservoirs in ART-Suppressed Human Lymph NodesResearch SquareJanuary 7, 2026Merantha Ndhlovu, Merantha Moodley, Caroline Hossain, Caroline Chasara, Bongiwe Mahlobo, Bongiwe Mahlobo, Kavidha Reddy, Thandekile Ngubane, Thandekile Pansegrouw, Crystal A. Pansegrouw, et al.immunoregulatory macrophages (36). HIV Gagp24 detected infected cells, and Ki67 marked active GCs. The Lunaphore COMET technology was used for these analyses, as it allows for high-plex multicolor immunofluorescence panel

(10) Macrophage-Dendritic Cell-T-Cell Tetrads Orchestrate Antitumor Immunity and Response to Checkpoint BlockadebioRxivJanuary 2, 2026Mehdi Chaib, Muhammad Aminu, Shelley Herbrich, Mahshid Arabi, Yue Xuan, Akshay Basi, Anna Casasent, Marc D. Macaluso, Kenneth H. Hu, Matthew Gubin, et al.tumors To visualize APC-T cell interactions in tumors, we used multiplexed imaging platforms (Lunaphore COMET and RNAscope), which enable simultaneous visualization of up to 20 protein and 12 RNA targets within

(11) The temporal gene expression landscape of rhabdomyolysis-induced acute kidney injury reveals the timing of complement activationCommunications BiologyDecember 31, 2025Anne Grunenwald, Idris Boudhabhay, Margot Revel, Victoria Poillerat, Elodie Voilin, Amine Majdi, Khalil Chaibi, Stephane Gaudry, Trent M. Woodruff, Gilles Crambert, et al.kidney sections from 2 mice injected with glycerol and 2 mice injected with PBS were examined using the COMET platform (Lunaphore Technologies). Hyperplex immunofluorescence imaging, applying our in situ Complementomics

(12) Society for Immunotherapy of Cancer: Standards for Reporting of Multiplex Immunohistochemistry/Immunofluorescence Assays (STORMI)J Immunother CancerDecember 21, 2025Sam Sater, Carlo B Bifulco, Jaime Rodriguez-Canales, Joe Yeong, Guray Akturk, Michael Angelo, Carmen Ballesteros-Merino, Peter Bankhead, Subham Basu, Jorge M Blando, et al.BioPharma, Carlsbad, CA, USA).”12“Automated hyperplex IF staining and imaging was performed on the COMET™ platform (Lunaphore Technologies) … The hyperplex immunocytochemistry protocol template was generated

(13) RIPK1 inhibition reduces biliary injury and fibrosis in primary sclerosing cholangitisSci AdvDecember 19, 2025Pierre-Antoine Soret, Virginie Steunou, Julien Hedou, Juliette Tokgozoglu, Valeria Pistorio, Amine Majdi, Nadia Darwane, Jean-Louis Delaunay, Siham Benyahia, Laetitia Dinard, et al.apoptosis is parenchymal.(A) Representative multiplex immunofluorescence imaging using the Lunaphore COMET platform from liver biopsy of patient #1 with PSC, stained for CYP2E1 (hepatocytes), α-SMA (smooth muscle cells

(14) High-Dimensional Single-Cell Analysis Reveals Coordinated Age-Dependent Neuroinflammatory Microglia-T cell Circuits in the BrainbioRxivDecember 13, 2025Md Akkas Ali, Md Hasanul Banna Siam, Donald Vardaman, Chase Bolding, J. Nicholas Brazell, Ashleigh D. Whatley, Christopher A. Risley, Harrison Tidwell, Syed Nakib Hossain, Juhi Samal, et al.captured in our SPLiT-seq analysis, we performed multiplex immunofluorescent imaging using Lunaphore COMET™. We stained tissue sections and performed COMET imaging for major immune cells including microglia,

(15) PixlMap: A generalisable pixel classifier for cellular phenotyping in multiplex immunofluorescence imagesPLoS OneDecember 3, 2025Rachel L. Baird, David Mason, Kai Rakovic, Fiona Ballantyne, Ian R. Powley, Anastasia Georgakopoulou, Lucy Hillary, Thomas G. Bird, Leah Officer-Jones, John Le Quesnetraining on images generated by alternative staining and imaging platforms (Akoya PhenoCycler, Lunaphore COMET) due to the difference in autofluorescence removal in these imaging modalities [33–35]. Each of these

(16) The effect of therapeutic potential and safety of bone marrow-derived against adipose-derived mesenchymal stem cells in aged mice associated with septic arthritisPLOS OneNovember 26, 2025Alani Mohanad Khalid Ahmed, Mujahid Khalaf Ali, Basma Kh. Alanito this, advanced imaging techniques such as multiplex imaging and immunohistochemistry (Lunaphore COMET™) were utilized to visualize the engraftment of MSCs and their interactions with host tissues at the

(17) Bi-specific T cell-engaging antibody triggers protective immune memory and glioma microenvironment remodeling in immune-competent preclinical modelsJ Immunother CancerOctober 28, 2025Markella Zannikou, Joseph T Duffy, Daniele Procissi, Hinda Najem, Rebecca N Levine, Aditi Thakur, Dolores Hambardzumyan, Catalina Lee-Chang, Lara Leoni, Craig M Horbinski, et al.immunofluorescence staining and imaging were performed on formalin fixed paraffin embedded (FFPE) sections using the COMET system (Lunaphore Technologies). The following targets and the associated antibodies were used for this

(18) From Histology to High-Resolution Mapping: The Rise of Spatial Omics in ImmunologyEur J ImmunolOctober 6, 2025Marco De Giovanni, Donato Inverso, Matteo Iannaconefunctional tissue analysis. Additional antibody‐based proteomics methods have been developed. For instance, COMET, a spatial proteomics approach from Lunaphore, uses microfluidics‐based sequential immunofluorescence

(19) Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardiumNat Cardiovasc ResOctober 3, 2025Florian Wünnemann, Florian Sicklinger, Kresimir Bestak, Jose Nimo, Tobias Thiemann, Junedh M. Amrute, Mathias Nordbeck, Niklas Hartmann, Miguel A. Ibarra-Arellano, Jovan Tanevski, et al.barcoding (Molecular Cartography) and antibody-based Sequential Immunofluorescence (SeqIF, Lunaphore COMET), respectively, to characterize the changing tissue microenvironment during acute MI in mice. Subsequently,

(20) Selective Alanine Transporter Utilization Is a Therapeutic Vulnerability in ARID1A-Mutant Ovarian CancerCancer ResearchSeptember 15, 2025Hao Nie, Liping Liao, Rafal J. Zielinski, Javier A. Gomez, Akshay V. Basi, Erin H. Seeley, Lin Tan, Agnes Julia Bilecz, Wei Zhou, Heng Liu, et al.modalities. Sequential Immunofluorescence Staining (seqIF) and Image analysis SeqIF was performed using the COMET instrument (Lunaphore Technologies) as previously described (30,31). Briefly, FFPE Tissue Microarray

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COMET ™

COMET ™

COMET ™ from Bio-Techne Spatial

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Bio-Techne Spatial

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Can your existing IHC or IF assays scale into high-plex spatial proteomics?

Spatial multiomics for comprehensive interrogation of the immune landscape and its activation states in cancer

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