Blocking Buffers and Reagents

Blocking buffers and reagents are commonly used for immunodetection applications such as ELISA, Western blot, and immunohistochemistry. Blockers prevent the non-specific binding of antibodies, ensuring that the signal detected comes from the target analytes. Effective use of blocking buffers minimizes background noise as well as long-term preservation of samples. ELISA blocking buffers can help stabilize the plates for drying and storage, minimizing signal loss. Mixed reagents that help to reduce non-specific binding of primary antibodies and increase the storage time of the primary antibody working solution during Western blots are also available. Common reagents used in protein blocking include milk, casein, serum, and BSA. In nucleic acid hybridization and blotting applications, genomic DNA from calf thymus and herring or salmon sperm, have been used. Specialty reagents such as avidin/biotin blockers block all endogenous biotin and avidin binding activity. Browse the products below and visit the reagent supplier page for more information.