Blocking buffers and reagents are commonly used for immunodetection applications such as ELISA, Western blot, and immunohistochemistry. Blockers prevent the non-specific binding of antibodies, ensuring that the signal detected comes from the target analytes. Effective use of blocking buffers minimizes background noise as well as long-term preservation of samples. ELISA blocking buffers can help stabilize the plates for drying and storage, minimizing signal loss. Mixed reagents that help to reduce non-specific binding of primary antibodies and increase the storage time of the primary antibody working solution during Western blots are also available. Common reagents used in protein blocking include milk, casein, serum, and BSA. In nucleic acid hybridization and blotting applications, genomic DNA from calf thymus and herring or salmon sperm, have been used. Specialty reagents such as avidin/biotin blockers block all endogenous biotin and avidin binding activity. Browse the products below and visit the reagent supplier page for more information.
Your search returned 30 Blocking Buffers and Reagents across 1 supplier.
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- Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a ...
- 100 mL
- Inquire
- Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western ...
- 80 g
- ELISA (EIA), Western Blot (WB)
- Heterophilic Antibody (HA) interference in double-antibody sandwich reaction models can lead to false positive results ...
- 50 mg
- ELISA (EIA), Double-antibody immunoassays
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- MBS238295 is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and ...
- 0.1 mg
- Flow cytometry (FC/FACS)
- MBS238295 is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and ...
- 0.5 mg
- Flow cytometry (FC/FACS)
- • Superior blocking compared to dry milk or serum
• Enhances low level phosphoprotein signal without ... - 4 l
- Inquire
- • Superior blocking compared to dry milk or serum
• Enhances low level phosphoprotein signal without ... - 1 l
- Inquire
- Serum Diluent Buffer
- 500 ml
- Immunoassay (IA)
- Ready-to-use biotin free reagent applicable for blocking ELISA plates
Ready-to-use biotin free reagent applicable for ... - 100 ml
- ELISA (EIA)
- 5 mL
- Western Blot (WB), Immunohistochemistry (IHC)
- Use Antibody Coating Buffer to save reagents, enhance specific signal, and extend the shelf life of coated plates. ...
- 100 mL
- Inquire
- Diluent buffer/blocker for use in Poly-HRP enhanced enzymatic labelling of antibodies and antigens
Conjugate Diluent/... - 100 ml
- Inquire
- Antigen Coating Buffer maximizes the adsorption of antigens onto polystyrene plates and stabilizes their structure to ...
- 100 mL
- Inquire
- Receive our three most popular Blocking Buffer formulations (General Block, Neptune Block, SynBlock) in this economical ...
- 1 Pack
- Inquire
- Blocking Buffer is intended to block non-specific
- 10 mL
- Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- Effective blocking agent used to increase signal-to-noise ratio for detection during hybridization
- 5 g
- Inquire
- Enhanced Universal Diluent/Blocker/Stabilizer for use in IHC
- 15 mL
- Immunohistochemistry (IHC)
- Monster Block ELISA Blocking Buffer is a high strength blocking buffer designed to address high
- 100 mL
- Inquire
- Neptune Block Blocking Buffer is non-mammalian and designed primarily for antigen-down ELISA formats as well as sandwich...
- 100 mL
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- Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference ...
- 100 mL
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- Eliminate interference and nonspecific
- 100 mL
- Inquire
- Universal Diluent/Blocker/Stabilizer for use in IHC
- 50 mL
- Immunohistochemistry (IHC)
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