Nitrocellulose membranes are used for protein and immunoblotting blotting techniques such as Western blot. Hydrophobic interactions help with protein immobilization on nitrocellulose membranes, while low methanol and high salt concentrations can improve the process. Nitrocellulose is favored due to its high protein-binding affinity, ability to immobilize proteins, glycoproteins, and amino acids, as well as its compatibility with detection methods such as fluorescence and chemiluminescence. Nitrocellulose has a protein binding capacity of 80 to 100 µg/cm2 and is therefore not as sensitive as PVDF membranes for detecting proteins with low expression. However, nitrocellulose membranes provide lower background noise. Due to their more brittle nature, these membranes require more care when stripping and reprobing to prevent signal loss. Nitrocellulose membranes are best for the detection of proteins of low molecular weight. Varying pore sizes are available and should be chosen based on the application. Visit the supplier page for more product information.
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