TR-FRET Assay Kits

TR-FRET assay kits are available to measure the amount or activity of a protein or biomolecule through the combined use of time-resolved (TR) measurement of fluorescence and fluorescence resonance energy transfer (FRET). FRET relies on the excitation and emission of two fluorophores that come into close proximity due to biomolecular interactions, such as from protein-ligand binding or enzyme-substrate interaction. Meanwhile, TR fluorescence uses lanthanide fluorophores to reduce short-lived background fluorescence, thus minimizing background noise. TR-FRET assays are useful in detecting enzyme activity, measuring enzyme inhibition, or quantifying substrates or ligands. Visit the assay supplier page for more information, including assay target and application.