BCA Assay Kits

BCA assay kits contain the reagents needed to measure total protein concentrations using the bicinchoninic acid (BCA) method. The assay, which relies on BCA, copper, and alkaline conditions, leads to the formation of complexes between copper ions and polypeptides. The residues that contribute to the reduction of Cu2+ include the cysteine, cystine, tryptophan, tyrosine, and the peptide bonds. The assay forms an intense purple solution that can be measured at 562 nm. BCA assays are highly sensitive, reliable, and can be used to determine yields in crude extracts and purified protein samples. They can also be used to detect protein contamination. While BCA assays can tolerate a small amount of detergents, they are temperature-sensitive and may also be affected by reducing agents and copper chelators. Visit the BCA assay kit suppliers for more information, including kit components and protocols.