
Kinase assay kits are available from a number of suppliers to measure the enzymatic activity of specific kinases in a biological sample. Phosphorylation by various kinases is important in many biological processes, including enzyme activation, regulation, metabolism, cell signaling, cell division, and more. Kinase assays generally incorporate the addition of specific kinase substrates into a reaction and measure the amount of products formed. Immunoassay-based kits utilize specific peptide sequences and corresponding antibodies that recognize the phosphorylated products. Colorimetric, fluorometric, and luminescent detection methods are available. Such assay kits can be useful in measuring the activity of pure and partially purified kinases, as well as in screening for kinase inhibitors. Choosing the appropriate kinase assay for your needs depends largely on the source of the kinase activity, available instrumentation, detection method, and the desired throughput. Visit the kinase assay kit suppliers for more details, including kit components.
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- Kinases (also called phosphotransferases) constitute a large class of enzymes that transfer phosphate groups from the ...
- 400 tests
- Fast and uniform protein kinase activity determination and ...
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- The kit contains purified recombinant Tie2 kinase, kinase substrate, ATP, assay buffer, and a white 96-well plate. This ...
- 96 reactions
- Kit is used to measure the activity of Tie2 kinase in a cell...
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