IMDM, or Iscove’s Modified Dulbecco’s Medium, was first developed by N.N. Iscove (1978) to successfully culture mouse B-lymphocytes to maturation without added serum. In the original IMDM recipe, Dulbecco’s Medium (DMEM) was enriched with selenium and serum replacements that consist of albumin, transferrin, and soybean lipids. Compared to other basal media, IMDM is highly enriched, containing greater amounts of amino acids, vitamins, and glucose. It has been reported to support various mammalian cells and tissue such as bone marrow, hematopoietic progenitors, and some cell lines.
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