Magnesium chloride (MgCl2) is a soluble compound and a necessary reagent in PCR-based experiments. Magnesium ions bind dNTPs; thus optimal MgCl2 concentration will depend on the amount of dNTPs. DNA polymerase also requires free magnesium as a cofactor. For a standard reaction with Taq polymerase, a working MgCl2 concentration of 1.5 to 2.0 mM is typically used. Excessive magnesium stabilizes DNA double-strand formation, but can also promote primer annealing to incorrect template sites, leading to the formation of incorrect products. Inadequate magnesium will result in reduced yield. Magnesium chloride can come supplied as a pure powder or in solution.
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- 1 EA Poly Bottle; 500g
- Magnesium Chloride Hexahydrate; Certified ACS; Assay: 99.0-102.0%; Crystalline; Fisher Chemical; 500g; Poly Bottle; F.W. 203.31; MgCl2--6H2O; CAS: ...
- 1 EA 5 x 10mL
- Media, Prepared; 2M Magnesium Chloride; Fisher BioReagents; Microbial Cell Culture Grade; For addition to SOB and SOC medium for propagating bacterial...
- 1 EA Poly Pail; 3kg
- Magnesium Chloride Hexahydrate; Certified ACS; Crystalline; Fisher Chemical; 3kg; Poly Pail; F.W. 203.31; MgCl2--6H2O; CAS: 7791-18-6
- 1 EA Poly Bottle; 500g
- Magnesium Chloride Hexahydrate; USP/FCC; Crystalline; Fisher Chemical; 500g; Poly Bottle; F.W. 203.31; MgCl2--6H2O; CAS: 7791-18-6
- 1 EA Poly Bottle; 2.5kg
- Magnesium Chloride Hexahydrate; USP/FCC; Crystalline; Fisher Chemical; 2.5kg; Poly Bottle; F.W. 203.31; MgCl2--6H2O; CAS: 7791-18-6
- 1 EA Poly Pail; 12kg
- Magnesium Chloride Hexahydrate; USP/FCC; Crystalline; Fisher Chemical; 12kg; Poly Pail; F.W. 203.31; MgCl2--6H2O; CAS: 7791-18-6
- 1 EA Poly Bottle; 2.5kg
- Magnesium Chloride Hexahydrate; USP/FCC/EP/BP; Crystalline; Fisher Chemical; 2.5kg; Poly Bottle; F.W. 203.31; MgCl2--6H2O; CAS: 7791-18-6
- 1 EA Poly Bottle; 500g
- Magnesium Chloride Hexahydrate; 500g; Poly Bottle; F.W. 203.30; MgCl2.6H2O; CAS: (7791-18-6)
- 100 ml
- Magnesium Chloride Solution
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- MgCl2 can be used as the sample diluent when attempting to overcome inhibitory chelation effect. Examples of samples which chelate divalent cations ...
- 25ml
- Magnesium Chloride Solution, supplied at 25mM, is used with GoTaq Flexi Reaction Buffers for PCR optimization
- 1.5ml
- Magnesium Chloride Solution, supplied at 25mM, is used with GoTaq Flexi Reaction Buffers for PCR optimization
- 6 ml
- 25 mM MgCl2 for use in conjunction with polymerase reaction buffers.
$26.00
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