Master mixes for qPCR contain the necessary reagents for a straightforward quantitative PCR or real-time PCR reaction. A given mix will typically include the polymerase, buffer, dNTPs, MgCl2, and fluorescent dye. In concentrations from 2X to 5X, it is ready-to-use with the addition of template and primers. For RNA templates, RT-qPCR kits containing reverse transcriptase are available in “one-step” or “two-step” formats. For specific details on reaction components, including the type of fluorophore and polymerase, visit the supplier pages.
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- Universal mix for qPCR using an intercalating dye that only detect double stranded DNA with high sensitivity
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- Universal mix for qPCR using an intercalating dye with fluorescein
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- High fidelity Hot Start DNA polymerase
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- Quick ligation of DNA fragments in 5 min at room temperature, ready-to-use solution
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- Hot Start polymerase master mix
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- One-Step Probe Mix with UDG for probe-based detection and quantification of RNA targets
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- Ready-to-use mix for reverse transcription with a modified RNase H domain and higher thermal stability, optimal choice for more complex applications, ...
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- Ready-to-use mix with Oligo dTs for reverse transcription with a modified RNase H domain and higher thermal stability, optimal choice for more complex...
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- Ready-to-use mix for reverse transcription with a modified RNase H domain and higher thermal stability, optimal choice for more complex applications, ...
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- Ready-to-use Taq polymerase master mix
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