qPCR Master Mixes

Master mixes for qPCR contain the necessary reagents for a straightforward quantitative PCR or real-time PCR reaction. A given mix will typically include the polymerase, buffer, dNTPs, MgCl2, and fluorescent dye. In concentrations from 2X to 5X, it is ready-to-use with the addition of template and primers. For RNA templates, RT-qPCR kits containing reverse transcriptase are available in “one-step” or “two-step” formats. For specific details on reaction components, including the type of fluorophore and polymerase, visit the supplier pages.