Kits and mixes for qPCR (quantitative PCR or real-time PCR) are available from many sources for studies in gene expression. The driving principle of qPCR is the fluorescent detection of cDNA amplification in real-time. The general components of a qPCR reaction accordingly include DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers, intercalating dye, reference dye, primers, and the cDNA template. For RNA templates, one-step or two-step qPCR kits will include a reverse transcriptase (also known as RT-qPCR kits). Visit the supplier details page to ensure that the kit is compatible with the intended qPCR experiment.
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- varies: 1 mL, 5 mL, 2 x 5 mL, 5 x 5 mL, 10 x 5 mL, 50 mL
- PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–...
- Expression, PCR, qPCR, Other
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- varies: 1 x 1 mL, 1 x 5 mL, 2 x 5 mL, 5 x 5 mL, 10 x 5 mL, 1...
- TaqMan Fast Advanced Master Mix is optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications, including ...
- Expression, PCR, qPCR, RNA Analysis, Other
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- varies: 5 x 1 mL, 1 x 10 mL, 1 x 1 mL
- TaqMan Fast Virus 1-Step Master Mix is a 4X formulation ideal for detection of both RNA and DNA viruses and multiplex gene expression studies, even in...
- Expression, PCR, qPCR, Other
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- varies: L (2900 reactions/967 µL), made to order, S (...
- TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a...
- Expression, PCR, qPCR, Other
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Thermo Fisher Scientific
Thermo Fisher Scientific