Description
TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end. Features of TaqMan Gene Expression assays include: • Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required • Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay • Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets • Accurate—identify small fold-changes with high accuracy of quantitation • Extensive content—Over 5 million predesigned assays available for over 25 different species • Gold-standard TaqMan qPCR chemistry draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology • Fast delivery—your assays shipped in as few as 1–2 days