Cell line cross-contamination or misidentification can lead to wasted time and funds, and even lamentable publication retractions. This critical issue has since prompted funding agencies, such as the National Institutes of Health, and a growing list of reputable publishers to put in place requirements to ensure authenticity and purity of involved cell lines. One well-established method, STR (short tandem repeat) analysis, determines the strength of a paired match by comparing key genomic loci in comparison to donor or database profiles. Other methods may include SNP analysis, DNA barcoding, and detection for mycoplasma. Based on these methods, cell authentication services are available, and may serve in saving time and effort while ensuring the true identity of your cells.
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- Cell Line Authentication, STR Analysis
- Nucleic acid extraction, PCR-based STR Analysis
- Human Cell Lines
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